Activation of PPARγ coactivator-1 through transcription factor docking

Pere Puigserver, Guillaume Adelmant, Zhidan Wu, Melina Fan, Jianming Xu, Bert O'Malley, Bruce M. Spiegelman

Research output: Contribution to journalArticle

Abstract

Transcriptional coactivators have been viewed as constitutively active components, using transcription factors mainly to localize their functions. Here, it is shown that PPARγ coactivator-1 (PGC-1) promotes transcription through the assembly of a complex that includes the histone acetyltransferases steroid receptor coactivator-1 (SRC-1) and CREB binding protein (CBP)/p300. PGC-1 has a low inherent transcriptional activity when it is not bound to a transcription factor. The docking of PGC-1 to peroxisome proliferator-activated receptor (PPARγ) stimulates an apparent conformational change in PGC-1 that permits binding of SRC-1 and CBP/p300, resulting in a large increase in transcriptional activity. Thus, transcription factor docking switches on the activity of a coactivator protein.

Original languageEnglish (US)
Pages (from-to)1368-1371
Number of pages4
JournalScience
Volume286
Issue number5443
DOIs
StatePublished - Nov 12 1999
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Puigserver, P., Adelmant, G., Wu, Z., Fan, M., Xu, J., O'Malley, B., & Spiegelman, B. M. (1999). Activation of PPARγ coactivator-1 through transcription factor docking. Science, 286(5443), 1368-1371. https://doi.org/10.1126/science.286.5443.1368