Activation of microglia by Borna disease virus infection: In vitro study

Mikhail V. Ovanesov, Christian Sauder, Steven A. Rubin, Jürgen Richt, Avindra Nath, Kathryn M. Carbone, Mikhail Pletnikov

Research output: Contribution to journalArticle

Abstract

Neonatal Borna disease virus (BDV) infection of the rat brain is associated with microglial activation and damage to the certain neuronal populations. Since persistent BDV infection of neurons in vitro is noncytolytic and noncytopathic, activated microglia have been suggested to be responsible for neuronal cell death in vivo. However, the mechanisms of activation of microglia in neonatally BDV-infected rat brain have not been investigated. To address these issues, activation of primary rat microglial cells was studied following exposure to purified BDV or to persistently BDV-infected primary cortical neurons or after BDV infection of primary mixed neuron-glial cultures. Neither purified virus nor BDV-infected neurons alone activated primary microglia as assessed by the changes in cell shape or production of the proinflammatory cytokines. In contrast, in the BDV-infected primary mixed cultures, we observed proliferation of microglia cells that acquired the round morphology and expressed major histocompatibility complex molecules of classes I and II. These manifestations of microglia activation were observed in the absence of direct BDV infection of microglia or overt neuronal toxicity. In addition, compared to uninfected mixed cultures, activation of microglia in BDV-infected mixed cultures was associated with a significantly greater lipopolysaccharide-induced release of tumor necrosis factor alpha, interleukin 1β, and interleukin 10. Taken together, the present data are the first in vitro evidence that persistent BDV infection of neurons and astrocytes rather than direct exposure to the virus or dying neurons is critical for activating microglia.

Original languageEnglish (US)
Pages (from-to)12141-12148
Number of pages8
JournalJournal of Virology
Volume80
Issue number24
DOIs
StatePublished - Dec 2006

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Borna disease virus
neuroglia
Microglia
Virus Diseases
in vitro studies
neurons
infection
Neurons
mixed culture
Infant, Newborn, Diseases
rats
In Vitro Techniques
Viruses
brain
viruses
Cell Shape
astrocytes
interleukin-1
Brain
major histocompatibility complex

ASJC Scopus subject areas

  • Immunology

Cite this

Ovanesov, M. V., Sauder, C., Rubin, S. A., Richt, J., Nath, A., Carbone, K. M., & Pletnikov, M. (2006). Activation of microglia by Borna disease virus infection: In vitro study. Journal of Virology, 80(24), 12141-12148. https://doi.org/10.1128/JVI.01648-06

Activation of microglia by Borna disease virus infection : In vitro study. / Ovanesov, Mikhail V.; Sauder, Christian; Rubin, Steven A.; Richt, Jürgen; Nath, Avindra; Carbone, Kathryn M.; Pletnikov, Mikhail.

In: Journal of Virology, Vol. 80, No. 24, 12.2006, p. 12141-12148.

Research output: Contribution to journalArticle

Ovanesov, MV, Sauder, C, Rubin, SA, Richt, J, Nath, A, Carbone, KM & Pletnikov, M 2006, 'Activation of microglia by Borna disease virus infection: In vitro study', Journal of Virology, vol. 80, no. 24, pp. 12141-12148. https://doi.org/10.1128/JVI.01648-06
Ovanesov MV, Sauder C, Rubin SA, Richt J, Nath A, Carbone KM et al. Activation of microglia by Borna disease virus infection: In vitro study. Journal of Virology. 2006 Dec;80(24):12141-12148. https://doi.org/10.1128/JVI.01648-06
Ovanesov, Mikhail V. ; Sauder, Christian ; Rubin, Steven A. ; Richt, Jürgen ; Nath, Avindra ; Carbone, Kathryn M. ; Pletnikov, Mikhail. / Activation of microglia by Borna disease virus infection : In vitro study. In: Journal of Virology. 2006 ; Vol. 80, No. 24. pp. 12141-12148.
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