Activation of endoplasmic reticulum stress response by enhanced polyamine catabolism is important in the mediation of cisplatin-induced acute kidney injury

Kamyar Zahedi, Sharon Barone, Christina Destefano-Shields, Marybeth Brooks, Tracy Murray Stewart, Matthew Dunworth, Weimin Li, Joanne R. Doherty, Mark A. Hall, Roger D. Smith, John L. Cleveland, Robert A Casero, Manoocher Soleimani

Research output: Contribution to journalArticle

Abstract

Cisplatin-induced nephrotoxicity limits its use in many cancer patients. The expression of enzymes involved in polyamine catabolism, spermidine/spermine N1-acetyltransferase (SSAT) and spermine oxidase (SMOX) increase in the kidneys of mice treated with cisplatin. We hypothesized that enhanced polyamine catabolism contributes to tissue damage in cisplatin acute kidney injury (AKI). Using gene knockout and chemical inhibitors, the role of polyamine catabolism in cisplatin AKI was examined. Deficiency of SSAT, SMOX or neutralization of the toxic products of polyamine degradation, H2O2 and aminopropanal, significantly diminished the severity of cisplatin AKI. In vitro studies demonstrated that the induction of SSAT and elevated polyamine catabolism in cells increases the phosphorylation of eukaryotic translation initiation factor 2α (eIF2α) and enhances the expression of binding immunoglobulin protein BiP/GRP78) and CCAAT-enhancer-binding protein homologous protein (CHOP/GADD153). The increased expression of these endoplasmic reticulum stress response (ERSR) markers was accompanied by the activation of caspase-3. These results suggest that enhanced polyamine degradation in cisplatin AKI may lead to tubular damage through the induction of ERSR and the consequent onset of apoptosis. In support of the above, we show that the ablation of the SSAT or SMOX gene, as well as the neutralization of polyamine catabolism products modulate the onset of ERSR (e.g. lower BiP and CHOP) and apoptosis (e.g. reduced activated caspase-3). These studies indicate that enhanced polyamine catabolism and its toxic products are important mediators of ERSR and critical to the pathogenesis of cisplatin AKI.

Original languageEnglish (US)
Article numbere0184570
JournalPLoS One
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2017

Fingerprint

cisplatin
Endoplasmic Reticulum Stress
Polyamines
polyamines
Acute Kidney Injury
spermine
endoplasmic reticulum
Cisplatin
stress response
Chemical activation
kidneys
acetyltransferases
Acetyltransferases
metabolism
Spermidine
Spermine
spermidine
Poisons
caspase-3
neutralization

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Activation of endoplasmic reticulum stress response by enhanced polyamine catabolism is important in the mediation of cisplatin-induced acute kidney injury. / Zahedi, Kamyar; Barone, Sharon; Destefano-Shields, Christina; Brooks, Marybeth; Murray Stewart, Tracy; Dunworth, Matthew; Li, Weimin; Doherty, Joanne R.; Hall, Mark A.; Smith, Roger D.; Cleveland, John L.; Casero, Robert A; Soleimani, Manoocher.

In: PLoS One, Vol. 12, No. 9, e0184570, 01.09.2017.

Research output: Contribution to journalArticle

Zahedi, K, Barone, S, Destefano-Shields, C, Brooks, M, Murray Stewart, T, Dunworth, M, Li, W, Doherty, JR, Hall, MA, Smith, RD, Cleveland, JL, Casero, RA & Soleimani, M 2017, 'Activation of endoplasmic reticulum stress response by enhanced polyamine catabolism is important in the mediation of cisplatin-induced acute kidney injury', PLoS One, vol. 12, no. 9, e0184570. https://doi.org/10.1371/journal.pone.0184570
Zahedi, Kamyar ; Barone, Sharon ; Destefano-Shields, Christina ; Brooks, Marybeth ; Murray Stewart, Tracy ; Dunworth, Matthew ; Li, Weimin ; Doherty, Joanne R. ; Hall, Mark A. ; Smith, Roger D. ; Cleveland, John L. ; Casero, Robert A ; Soleimani, Manoocher. / Activation of endoplasmic reticulum stress response by enhanced polyamine catabolism is important in the mediation of cisplatin-induced acute kidney injury. In: PLoS One. 2017 ; Vol. 12, No. 9.
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