Activation of canonical WNT/β-catenin signaling enhances in vitro motility of glioblastoma cells by activation of ZEB1 and other activators of epithelial-to-mesenchymal transition

Ulf D. Kahlert, Donata Maciaczyk, Soroush Doostkam, Brent A. Orr, Brian Simons, Tomasz Bogiel, Thomas Reithmeier, Marco Prinz, Jörg Schubert, Gabriele Niedermann, Thomas Brabletz, Charles G Eberhart, Guido Nikkhah, Jaroslaw Maciaczyk

Research output: Contribution to journalArticle

Abstract

Here we show that activation of the canonical WNT/β-catenin pathway increases the expression of stem cell genes and promotes the migratory and invasive capacity of glioblastoma. Modulation of WNT signaling alters the expression of epithelial-to-mesenchymal transition activators, suggesting a role of this process in the regulation of glioma motility. Using immunohistochemistry in patient-derived glioblastoma samples we showed higher numbers of cells with intranuclear signal for β-catenin in the infiltrating edge of tumor compared to central tumor parenchyma. These findings suggest that canonical WNT/β-catenin pathway is a critical regulator of GBM invasion and may represent a potential therapeutic target.

Original languageEnglish (US)
Pages (from-to)42-53
Number of pages12
JournalCancer Letters
Volume325
Issue number1
DOIs
StatePublished - Dec 1 2012

Fingerprint

Catenins
Epithelial-Mesenchymal Transition
Glioblastoma
Cell Movement
Glioma
Neoplasms
Stem Cells
Cell Count
Immunohistochemistry
Genes
In Vitro Techniques
Therapeutics

Keywords

  • β-Catenin
  • Epithelial-to-mesenchymal transition (EMT)
  • Glioblastoma
  • Invasion
  • ZEB1

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Activation of canonical WNT/β-catenin signaling enhances in vitro motility of glioblastoma cells by activation of ZEB1 and other activators of epithelial-to-mesenchymal transition. / Kahlert, Ulf D.; Maciaczyk, Donata; Doostkam, Soroush; Orr, Brent A.; Simons, Brian; Bogiel, Tomasz; Reithmeier, Thomas; Prinz, Marco; Schubert, Jörg; Niedermann, Gabriele; Brabletz, Thomas; Eberhart, Charles G; Nikkhah, Guido; Maciaczyk, Jaroslaw.

In: Cancer Letters, Vol. 325, No. 1, 01.12.2012, p. 42-53.

Research output: Contribution to journalArticle

Kahlert, UD, Maciaczyk, D, Doostkam, S, Orr, BA, Simons, B, Bogiel, T, Reithmeier, T, Prinz, M, Schubert, J, Niedermann, G, Brabletz, T, Eberhart, CG, Nikkhah, G & Maciaczyk, J 2012, 'Activation of canonical WNT/β-catenin signaling enhances in vitro motility of glioblastoma cells by activation of ZEB1 and other activators of epithelial-to-mesenchymal transition', Cancer Letters, vol. 325, no. 1, pp. 42-53. https://doi.org/10.1016/j.canlet.2012.05.024
Kahlert, Ulf D. ; Maciaczyk, Donata ; Doostkam, Soroush ; Orr, Brent A. ; Simons, Brian ; Bogiel, Tomasz ; Reithmeier, Thomas ; Prinz, Marco ; Schubert, Jörg ; Niedermann, Gabriele ; Brabletz, Thomas ; Eberhart, Charles G ; Nikkhah, Guido ; Maciaczyk, Jaroslaw. / Activation of canonical WNT/β-catenin signaling enhances in vitro motility of glioblastoma cells by activation of ZEB1 and other activators of epithelial-to-mesenchymal transition. In: Cancer Letters. 2012 ; Vol. 325, No. 1. pp. 42-53.
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