Activated signal transducer and activator of transcription (STAT) 3: Localization in focal adhesions and function in ovarian cancer cell motility

Debra L. Silver, Honami Naora, Jinsong Liu, Wenjun Cheng, Denise J. Montell

Research output: Contribution to journalArticle

Abstract

Constitutive activation of the Janus-activated kinase/signal transducer and activator of transcription (STAT) pathway promotes the proliferation and survival of cancer cells in culture and is associated with various cancers, including those of the ovary. We found that constitutively activated STAT3 levels correlated with aggressive clinical behavior of ovarian carcinoma specimens. Furthermore, inhibition of STAT3 reduced the motility of ovarian cancer cells in vitro. Surprisingly, we found that activated STAT3 localized not only to nuclei but also to focal adhesions in these cells. Activated STAT3 coimmunoprecipitated with phosphorylated paxillin and focal adhesion kinase and required paxillin and Src for its localization to focal adhesions. These results suggest that Janus-activated kinase/STAT signaling may contribute to ovarian cancer cell invasiveness.

Original languageEnglish (US)
Pages (from-to)3550-3558
Number of pages9
JournalCancer Research
Volume64
Issue number10
DOIs
StatePublished - May 15 2004

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STAT3 Transcription Factor
Focal Adhesions
Ovarian Neoplasms
Paxillin
Cell Movement
Janus Kinases
Transducers
Focal Adhesion Protein-Tyrosine Kinases
Ovary
Neoplasms
Cell Culture Techniques
Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Activated signal transducer and activator of transcription (STAT) 3 : Localization in focal adhesions and function in ovarian cancer cell motility. / Silver, Debra L.; Naora, Honami; Liu, Jinsong; Cheng, Wenjun; Montell, Denise J.

In: Cancer Research, Vol. 64, No. 10, 15.05.2004, p. 3550-3558.

Research output: Contribution to journalArticle

Silver, Debra L. ; Naora, Honami ; Liu, Jinsong ; Cheng, Wenjun ; Montell, Denise J. / Activated signal transducer and activator of transcription (STAT) 3 : Localization in focal adhesions and function in ovarian cancer cell motility. In: Cancer Research. 2004 ; Vol. 64, No. 10. pp. 3550-3558.
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