Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

Meinrad Gawaz; Franz Josef Neumann; Timm Dickfeld; Werner Koch; Karl Ludwig Laugwitz; Helmut Adelsberger; Kirsten Langenbrink; Sharon Page; Dieter Neumeier; Albert Schömig; Korbinian Brand

Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

Meinrad Gawaz, Franz Josef Neumann, Timm Dickfeld, Werner Koch, Karl Ludwig Laugwitz, Helmut Adelsberger, Kirsten Langenbrink, Sharon Page, Dieter Neumeier, Albert Schömig, Korbinian Brand

Research output: Contribution to journal › Article › peer-review

249 Scopus citations

Abstract

Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

Original language	English (US)
Pages (from-to)	1164-1171
Number of pages	8
Journal	Circulation
Volume	98
Issue number	12
State	Published - Sep 22 1998
Externally published	Yes

Keywords

Cell adhesion molecules
Endothelium
Genes
Platelets
Proteins

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine

Cite this

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title = "Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells",

abstract = "Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P",

keywords = "Cell adhesion molecules, Endothelium, Genes, Platelets, Proteins",

author = "Meinrad Gawaz and Neumann, {Franz Josef} and Timm Dickfeld and Werner Koch and Laugwitz, {Karl Ludwig} and Helmut Adelsberger and Kirsten Langenbrink and Sharon Page and Dieter Neumeier and Albert Sch{\"o}mig and Korbinian Brand",

year = "1998",

month = sep,

day = "22",

language = "English (US)",

volume = "98",

pages = "1164--1171",

journal = "Circulation",

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publisher = "Lippincott Williams and Wilkins",

number = "12",

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TY - JOUR

T1 - Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

AU - Gawaz, Meinrad

AU - Neumann, Franz Josef

AU - Dickfeld, Timm

AU - Koch, Werner

AU - Laugwitz, Karl Ludwig

AU - Adelsberger, Helmut

AU - Langenbrink, Kirsten

AU - Page, Sharon

AU - Neumeier, Dieter

AU - Schömig, Albert

AU - Brand, Korbinian

PY - 1998/9/22

Y1 - 1998/9/22

N2 - Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

AB - Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

KW - Cell adhesion molecules

KW - Endothelium

KW - Genes

KW - Platelets

KW - Proteins

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M3 - Article

C2 - 9743506

AN - SCOPUS:0032558466

SN - 0009-7322

VL - 98

SP - 1164

EP - 1171

JO - Circulation

JF - Circulation

IS - 12

ER -

Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

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