Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

Meinrad Gawaz, Franz Josef Neumann, Timm Dickfeld, Werner Koch, Karl Ludwig Laugwitz, Helmut Adelsberger, Kirsten Langenbrink, Sharon Page, Dieter Neumeier, Albert Schömig, Korbinian Brand

Research output: Contribution to journalArticlepeer-review

Abstract

Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

Original languageEnglish (US)
Pages (from-to)1164-1171
Number of pages8
JournalCirculation
Volume98
Issue number12
StatePublished - Sep 22 1998
Externally publishedYes

Keywords

  • Cell adhesion molecules
  • Endothelium
  • Genes
  • Platelets
  • Proteins

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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