Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells

Meinrad Gawaz, Franz Josef Neumann, Timm Dickfeld, Werner Koch, Karl Ludwig Laugwitz, Helmut Adelsberger, Kirsten Langenbrink, Sharon Page, Dieter Neumeier, Albert Schömig, Korbinian Brand

Research output: Contribution to journalArticle

Abstract

Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

Original languageEnglish (US)
Pages (from-to)1164-1171
Number of pages8
JournalCirculation
Volume98
Issue number12
StatePublished - Sep 22 1998
Externally publishedYes

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Chemokine CCL2
Intercellular Adhesion Molecule-1
Blood Platelets
Endothelial Cells
Adenosine Diphosphate
Human Umbilical Vein Endothelial Cells
Endothelium
Atherosclerosis
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Cell adhesion molecules
  • Endothelium
  • Genes
  • Platelets
  • Proteins

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Gawaz, M., Neumann, F. J., Dickfeld, T., Koch, W., Laugwitz, K. L., Adelsberger, H., ... Brand, K. (1998). Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells. Circulation, 98(12), 1164-1171.

Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells. / Gawaz, Meinrad; Neumann, Franz Josef; Dickfeld, Timm; Koch, Werner; Laugwitz, Karl Ludwig; Adelsberger, Helmut; Langenbrink, Kirsten; Page, Sharon; Neumeier, Dieter; Schömig, Albert; Brand, Korbinian.

In: Circulation, Vol. 98, No. 12, 22.09.1998, p. 1164-1171.

Research output: Contribution to journalArticle

Gawaz, M, Neumann, FJ, Dickfeld, T, Koch, W, Laugwitz, KL, Adelsberger, H, Langenbrink, K, Page, S, Neumeier, D, Schömig, A & Brand, K 1998, 'Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells', Circulation, vol. 98, no. 12, pp. 1164-1171.
Gawaz M, Neumann FJ, Dickfeld T, Koch W, Laugwitz KL, Adelsberger H et al. Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells. Circulation. 1998 Sep 22;98(12):1164-1171.
Gawaz, Meinrad ; Neumann, Franz Josef ; Dickfeld, Timm ; Koch, Werner ; Laugwitz, Karl Ludwig ; Adelsberger, Helmut ; Langenbrink, Kirsten ; Page, Sharon ; Neumeier, Dieter ; Schömig, Albert ; Brand, Korbinian. / Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells. In: Circulation. 1998 ; Vol. 98, No. 12. pp. 1164-1171.
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AU - Gawaz, Meinrad

AU - Neumann, Franz Josef

AU - Dickfeld, Timm

AU - Koch, Werner

AU - Laugwitz, Karl Ludwig

AU - Adelsberger, Helmut

AU - Langenbrink, Kirsten

AU - Page, Sharon

AU - Neumeier, Dieter

AU - Schömig, Albert

AU - Brand, Korbinian

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AB - Background - Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte Chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed. Methods and Results - Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM- 1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-1 secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P

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