Activated human platelets in plasma excite nociceptors in rat skin, in vitro

Matthias Ringkamp, M. Schmelz, M. Kress, M. Allwang, A. Ogilvie, P. W. Reeh

Research output: Contribution to journalArticle

Abstract

Extravascular activation of thrombocytes may contribute to nociceptor excitation and pain, since platelets store and, upon stimulation, release potential algogenic substances such as serotonin, histamine and precursor molecules of bradykinin. To test this hypothesis, a skin-nerve preparation of rat hairy skin, in vitro, was used that allows to record and characterize single afferent nerve fibers. In a first protocol, receptive fields of nociceptive C-fibers, at the corium side of the skin patch, were exposed to adenosine diphosphate (ADP), to heparinized human platelet-rich plasma (PRP) and to PRP activated by ADP. Such activated platelets excited 9 11 units characterized as mechano-heat responsive C-nociceptors (CMH); peak discharges of more than 10 spikes/s were observed. After application of activated PRP, 4 5 high threshold mechanosensitive C-units and 4 5 mechano-cold sensitive C -units became responsive to heat stimulation but only few of these fibers were excited ( 1 5 in each group). In a second series of experiments the exposure to native PRP was prolonged to test for the effect of spontaneous platelet activation resulting from cutaneous collagen. Prolonged exposure did, but not significantly, enhance fiber discharge. During subsequent exposure to activated PRP, the discharge commenced, on average, after a significant delay of about three minutes. With this protocol 5 7 CMH units were driven by activated platelets. Following both protocols, mechanical (v.Frey) and thermal thresholds of the CMH units were not significantly altered. The findings demonstrate that nociceptors can indeed be driven and sensitized by activated platelets. This pain inducing mechanism may be relevant to certain clinical conditions, and it appears promising to scrutinize the chemical factors involved.

Original languageEnglish (US)
Pages (from-to)103-106
Number of pages4
JournalNeuroscience Letters
Volume170
Issue number1
DOIs
StatePublished - Mar 28 1994
Externally publishedYes

Fingerprint

Nociceptors
Platelet-Rich Plasma
Blood Platelets
Skin
Hot Temperature
Adenosine Diphosphate
Pain
Unmyelinated Nerve Fibers
Platelet Activation
Bradykinin
Dermis
Nerve Fibers
Histamine
Serotonin
Collagen
In Vitro Techniques

Keywords

  • CHM fiber
  • Inflammation
  • Pain
  • Polymodal
  • Sensitization
  • Thrombocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activated human platelets in plasma excite nociceptors in rat skin, in vitro. / Ringkamp, Matthias; Schmelz, M.; Kress, M.; Allwang, M.; Ogilvie, A.; Reeh, P. W.

In: Neuroscience Letters, Vol. 170, No. 1, 28.03.1994, p. 103-106.

Research output: Contribution to journalArticle

Ringkamp, Matthias ; Schmelz, M. ; Kress, M. ; Allwang, M. ; Ogilvie, A. ; Reeh, P. W. / Activated human platelets in plasma excite nociceptors in rat skin, in vitro. In: Neuroscience Letters. 1994 ; Vol. 170, No. 1. pp. 103-106.
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