Action of prokaryotic enhancer over a distance does not require continued presence of promoter-bound σ54 subunit

Vladimir Bondarenko, Ye Liu, Alexander Ninfa, Vasily M. Studitsky

Research output: Contribution to journalArticle

Abstract

The mechanism by which an enhancer activates transcription over large distances has been investigated. Activation of the glnAp2 promoter by the NtrC-dependent enhancer in Escherichia coli was analyzed using a purified system supporting multiple-round transcription in vitro. Our results suggest that the enhancer-promoter interaction and the initiation complex must be formed de novo during every round of transcription. No protein remained bound to the promoter after RNA polymerase escaped into elongation. Furthermore, the rate of initiation during the first and subsequent rounds of transcription were very similar, suggesting that there was no functional 'memory' facilitating multiple rounds of transcription. These studies exclude the hypothesis that enhancer action during multiple-round transcription involves the memory of the initial activation event.

Original languageEnglish (US)
Pages (from-to)636-642
Number of pages7
JournalNucleic Acids Research
Volume30
Issue number3
StatePublished - Feb 1 2002
Externally publishedYes

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DNA-Directed RNA Polymerases
Escherichia coli
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics

Cite this

Action of prokaryotic enhancer over a distance does not require continued presence of promoter-bound σ54 subunit. / Bondarenko, Vladimir; Liu, Ye; Ninfa, Alexander; Studitsky, Vasily M.

In: Nucleic Acids Research, Vol. 30, No. 3, 01.02.2002, p. 636-642.

Research output: Contribution to journalArticle

Bondarenko, Vladimir ; Liu, Ye ; Ninfa, Alexander ; Studitsky, Vasily M. / Action of prokaryotic enhancer over a distance does not require continued presence of promoter-bound σ54 subunit. In: Nucleic Acids Research. 2002 ; Vol. 30, No. 3. pp. 636-642.
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