TY - JOUR
T1 - Action of DCCD on the H+ O stoichiometry of mitoplast cytochrome c oxidase
AU - Lehninger, Albert L.
AU - Reynafarje, Baltazar
AU - Costa, Lidia
PY - 1985
Y1 - 1985
N2 - The mechanistic H+ O ejection stoichiometry of the cytochrome c oxidase reaction in rat liver miloplasts is close to 4 at level flow when the reduced oxidase is pulsed with O2. Dicyclohexylcarbodiimide (DCCD) up to 30 nmol mg protein fails to influence the rate of electron flow through the mitoplast oxidase, but inhibits H+ ejection. The inhibition of H+ ejection appears to be biphasic; ejection of 2-3 H+ per O is completely inhibited by very low DCCD, whereas inhibition of the remaining H+ ejection requires very much higher concentrations of DCCD. This effect suggests the occurrence of two types of H+ pumps in the native cytochrome oxidase of mitoplasts.
AB - The mechanistic H+ O ejection stoichiometry of the cytochrome c oxidase reaction in rat liver miloplasts is close to 4 at level flow when the reduced oxidase is pulsed with O2. Dicyclohexylcarbodiimide (DCCD) up to 30 nmol mg protein fails to influence the rate of electron flow through the mitoplast oxidase, but inhibits H+ ejection. The inhibition of H+ ejection appears to be biphasic; ejection of 2-3 H+ per O is completely inhibited by very low DCCD, whereas inhibition of the remaining H+ ejection requires very much higher concentrations of DCCD. This effect suggests the occurrence of two types of H+ pumps in the native cytochrome oxidase of mitoplasts.
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U2 - 10.1016/0162-0134(85)85043-1
DO - 10.1016/0162-0134(85)85043-1
M3 - Article
C2 - 2410565
AN - SCOPUS:0022036547
SN - 0162-0134
VL - 23
SP - 335
EP - 340
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
IS - 3-4
ER -