Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes

Hongbin Li, Fengli Guo, Boris Rubinstein, Rong Li

Research output: Contribution to journalArticle

Abstract

Movement of meiosis I (MI) chromosomes from the oocyte centre to a subcortical location is the first step in the establishment of cortical polarity. this is required for two consecutive rounds of asymmetric meiotic cell divisions, which generate a mature egg and two polar bodies. Here we use live-cell imaging and genetic and pharmacological manipulations to determine the forcegenerating mechanism underlying this chromosome movement. Chromosomes were observed to move toward the cortex in a pulsatile manner along a meandering path. this movement is not propelled by myosin-II-driven cortical flow but is associated with a cloud of dynamic actin filaments trailing behind the chromosomes/ spindle. Formation of these filaments depends on the actin nucleation activity of Fmn2, a formin-family protein that concentrates around chromosomes through its aminoterminal region symmetry breaking of the actin cloud relative to chromosomes, and net chromosome translocation toward the cortex require actin turnover.

Original languageEnglish (US)
Pages (from-to)1301-1308
Number of pages8
JournalNature Cell Biology
Volume10
Issue number11
DOIs
StatePublished - 2008
Externally publishedYes

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Oocytes
Actins
Chromosomes
Asymmetric Cell Division
Myosin Type II
Polar Bodies
Meiosis
Actin Cytoskeleton
Ovum
Pharmacology
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes. / Li, Hongbin; Guo, Fengli; Rubinstein, Boris; Li, Rong.

In: Nature Cell Biology, Vol. 10, No. 11, 2008, p. 1301-1308.

Research output: Contribution to journalArticle

Li, Hongbin ; Guo, Fengli ; Rubinstein, Boris ; Li, Rong. / Actin-driven chromosomal motility leads to symmetry breaking in mammalian meiotic oocytes. In: Nature Cell Biology. 2008 ; Vol. 10, No. 11. pp. 1301-1308.
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