ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

Eunsoo Lee, Jungyoon Choi, Youhwa Jo, Joo Yeon Kim, Yu Jin Jang, Hye Myeong Lee, So Yeun Kim, Ho Jae Lee, Keunchang Cho, Neoncheol Jung, Eun Mi Hur, Sung Jin Jeong, Cheil Moon, Youngshik Choe, Im Joo Rhyu, Hyun Kim, Woong Sun

Research output: Contribution to journalArticle

Abstract

Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies.

Original languageEnglish (US)
Article number18631
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 11 2016
Externally publishedYes

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Pressure
Three-Dimensional Imaging
Cellular Structures
Proteins
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Lee, E., Choi, J., Jo, Y., Kim, J. Y., Jang, Y. J., Lee, H. M., ... Sun, W. (2016). ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging. Scientific Reports, 6, [18631]. https://doi.org/10.1038/srep18631

ACT-PRESTO : Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging. / Lee, Eunsoo; Choi, Jungyoon; Jo, Youhwa; Kim, Joo Yeon; Jang, Yu Jin; Lee, Hye Myeong; Kim, So Yeun; Lee, Ho Jae; Cho, Keunchang; Jung, Neoncheol; Hur, Eun Mi; Jeong, Sung Jin; Moon, Cheil; Choe, Youngshik; Rhyu, Im Joo; Kim, Hyun; Sun, Woong.

In: Scientific Reports, Vol. 6, 18631, 11.01.2016.

Research output: Contribution to journalArticle

Lee, E, Choi, J, Jo, Y, Kim, JY, Jang, YJ, Lee, HM, Kim, SY, Lee, HJ, Cho, K, Jung, N, Hur, EM, Jeong, SJ, Moon, C, Choe, Y, Rhyu, IJ, Kim, H & Sun, W 2016, 'ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging', Scientific Reports, vol. 6, 18631. https://doi.org/10.1038/srep18631
Lee, Eunsoo ; Choi, Jungyoon ; Jo, Youhwa ; Kim, Joo Yeon ; Jang, Yu Jin ; Lee, Hye Myeong ; Kim, So Yeun ; Lee, Ho Jae ; Cho, Keunchang ; Jung, Neoncheol ; Hur, Eun Mi ; Jeong, Sung Jin ; Moon, Cheil ; Choe, Youngshik ; Rhyu, Im Joo ; Kim, Hyun ; Sun, Woong. / ACT-PRESTO : Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging. In: Scientific Reports. 2016 ; Vol. 6.
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