Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens

L. Lefrancois, L. Puddington, Carolyn E Machamer, M. J. Bevan

Research output: Contribution to journalArticle

Abstract

Cytotoxic T cell (CTL)-specific activation antigens, termed CT determinants, have been detected by monoclonal antibodies (mAb) that inhibit CTL function. At the cell surface, the CT antigens are associated with the T200 glycoproteins and two other proteins of M(r) 140,000 and 85,000 and are present on a secreted protein, gp155. Periodate treatment followed by binding analysis and immunoprecipitation experiments using tunicamycin-treated cells indicated that carbohydrate is necessary for CT antigen expression. Furthermore, gp155 is secreted in the presence of tunicamycin while retaining the CT antigens, and the CT determinants are added late in T200 biosynthesis, suggesting that the CT glycans are O-linked. Finally, interleukin 2 was shown to dramatically influence the expression of the CT mAb-reactive oligosaccharides present at the CTL cell surface.

Original languageEnglish (US)
Pages (from-to)1275-1293
Number of pages19
JournalJournal of Experimental Medicine
Volume162
Issue number4
StatePublished - 1985
Externally publishedYes

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Differentiation Antigens
Cytotoxic T-Lymphocytes
Tunicamycin
Carbohydrates
Antigens
CD27 Antigens
Monoclonal Antibodies
T-Lymphocytes
Oligosaccharides
Immunoprecipitation
Interleukin-2
Polysaccharides
Glycoproteins
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens. / Lefrancois, L.; Puddington, L.; Machamer, Carolyn E; Bevan, M. J.

In: Journal of Experimental Medicine, Vol. 162, No. 4, 1985, p. 1275-1293.

Research output: Contribution to journalArticle

Lefrancois, L. ; Puddington, L. ; Machamer, Carolyn E ; Bevan, M. J. / Acquisition of cytotoxic T lymphocyte-specific carbohydrate differentiation antigens. In: Journal of Experimental Medicine. 1985 ; Vol. 162, No. 4. pp. 1275-1293.
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