TY - JOUR
T1 - Acid Dissociation of Immune Complexes Improves Diagnostic Utility of p24 Antigen Detection in Perinatally Acquired Human Immunodeficiency Virus Infection
AU - Quinn, Thomas C.
AU - Kline, Richard
AU - Moss, Mark W.
AU - Livingston, Robert A.
AU - Hutton, Nancy
AU - Quinn, Thomas C.
AU - Kline, Richard
AU - Moss, Mark W.
AU - Livingston, Robert A.
AU - Hutton, Nancy
N1 - Funding Information:
Received 31 September 1992; revised 31 December 1992. Informed consent was obtained from patients' parents or guardians. The study was approved by the Johns Hopkins Joint Committee on Clinical Investigation. Grant support: National Institutes of Health (AI-27565, NR-02069, RR-0052). Reprints or correspondence: Dr. Thomas C. Quinn, Johns Hopkins University School of Medicine, Ross Research Bldg. Suite 1159. 720 Rutland Ave., Baltimore, MD 21205-2196.
PY - 1993/5
Y1 - 1993/5
N2 - Since acid treatment of serum is known to disrupt immune complexes, the diagnostic utility of the p24 antigen assay was examined after acid treatment of 345 serum samples from 158 children born to women infected with human immunodeficiency virus (HIV). Although the p24 antigen assay after acid treatment was negative in 9 HIV-1-infected children < 1 week old, antigen was detectable at high levels in all 30 samples obtained from infected children 1-9 months old. Overall, antigen was positive in 145 (sensitivity 89.5%) of 162 samples from 47 HIV-1-infected children ⩾1 month old. In contrast, the sensitivity of the p24 antigen assay without acid dissociation was only 18% (P <.001). Among the 76 uninfected children, 132 (specificity 99.2%) of 133 specimens were p24 antigen-negative after acid dissociation. These results demonstrate that acid treatment of serum markedly improves the sensitivity and predictive value of the p24 antigen assay for diagnosis of perinatally acquired HIV-1 infection in children 1 month of age or older.
AB - Since acid treatment of serum is known to disrupt immune complexes, the diagnostic utility of the p24 antigen assay was examined after acid treatment of 345 serum samples from 158 children born to women infected with human immunodeficiency virus (HIV). Although the p24 antigen assay after acid treatment was negative in 9 HIV-1-infected children < 1 week old, antigen was detectable at high levels in all 30 samples obtained from infected children 1-9 months old. Overall, antigen was positive in 145 (sensitivity 89.5%) of 162 samples from 47 HIV-1-infected children ⩾1 month old. In contrast, the sensitivity of the p24 antigen assay without acid dissociation was only 18% (P <.001). Among the 76 uninfected children, 132 (specificity 99.2%) of 133 specimens were p24 antigen-negative after acid dissociation. These results demonstrate that acid treatment of serum markedly improves the sensitivity and predictive value of the p24 antigen assay for diagnosis of perinatally acquired HIV-1 infection in children 1 month of age or older.
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U2 - 10.1093/infdis/167.5.1193
DO - 10.1093/infdis/167.5.1193
M3 - Article
C2 - 8486954
AN - SCOPUS:0027400918
SN - 0022-1899
VL - 167
SP - 1193
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -