@article{91aeaa1dde7c4d57891e70f5830ef54b,
title = "Acetylation of RNA Polymerase II Regulates Growth-Factor-Induced Gene Transcription in Mammalian Cells",
abstract = "Lysine acetylation regulates transcription by targeting histones and nonhistone proteins. Here we report that the central regulator of transcription, RNA polymerase II, is subject to acetylation in mammalian cells. Acetylation occurs at eight lysines within the C-terminal domain (CTD) of the largest polymerase subunit and is mediated by p300/KAT3B. CTD acetylation is specifically enriched downstream of the transcription start sites of polymerase-occupied genes genome-wide, indicating a role in early stages of transcription initiation or elongation. Mutation oflysines or p300 inhibitor treatment causes the loss of epidermal growth-factor-induced expression of c-Fos and Egr2, immediate-early genes with promoter-proximally paused polymerases, but does not affect expression or polymerase occupancy at housekeeping genes. Our studies identify acetylation as a new modification of the mammalian RNA polymerase II required for the induction of growth factor response genes.",
author = "Sebastian Schr{\"o}der and Eva Herker and Friederike Itzen and Daniel He and Sean Thomas and Gilchrist, {Daniel A.} and Katrin Kaehlcke and Sungyoo Cho and Pollard, {Katherine S.} and Capra, {John A.} and Martina Schn{\"o}lzer and Cole, {Philip A.} and Matthias Geyer and Bruneau, {Benoit G.} and Karen Adelman and Melanie Ott",
note = "Funding Information: We thank Jeffrey Corden and Andrew Rice for sharing reagents; Alexander Williams, Kirsten Eilertson, Brian Egan, Pao-Chen Li, and Ginger Muse for advice and assistance with experiments; and Gary Howard and Anna Lisa Lucido for editorial assistance. We gratefully acknowledge funds from Gladstone, the NIH (AI083139-02 [M.O.], GM62437 [P.A.C.], GM82901 [K.S.P. and J.A.C.], U01HL098179 [K.S.P. and B.G.B.]), the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences (K.A., Z01 ES101987), the Deutsche Forschungsgemeinschaft (M.G., GE 976/5), the Boehringer Ingelheim Fonds (S.S.), the Human Frontiers Science Program and an E.G.G. fellowship (E.H.), and the PhRMA Foundation (J.A.C.). ",
year = "2013",
month = nov,
day = "7",
doi = "10.1016/j.molcel.2013.10.009",
language = "English (US)",
volume = "52",
pages = "314--324",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}