Acceleration of G1cooperates with core binding factor β-smooth muscle myosin heavy chain to induce acute leukemia in mice

Yandan Yang, Weihua Wang, Rebecca Cleaves, Marianna Zahurak, Linzhao Cheng, Curt I. Civin, Alan D. Friedman

Research output: Contribution to journalArticle

Abstract

The genes encoding the AML1 (RUNX1) or CBFβ subunits of core binding factor (CBF) are commonly altered by translocation or mutation in human leukemias. Because CBF oncoproteins slow G1we sought to determine whether mutations that accelerate G1potentiate their ability to induce transformation. Wild-type or p16INK4ap19ARF(-/-) marrow cells transduced with CBFβ-smooth muscle myosin heavy chain (SMMHC) were transplanted into wild-type, syngeneic recipients. CBFβ-SMMHC significantly increased the development of acute leukemias from marrow lacking the overlapping p16p19 genes, based on analysis of Kaplan-Meier event-time distributions. Wild-type marrow was also transduced with vectors expressing either E7 alone or both E7 and CBFβ-SMMHC. Combining oncogenes again increased leukemia formation. Exposing mice transplanted with CBFβ-SMMHC-transduced cells to a mutagen, ethylnitrosourea, markedly accelerated leukemogenesis compared to expressing CBFβ-SMMHC with loss of p16p19, indicating the need for multiple “hits” for transformation. The INV/p16p19 and INV/E7 leukemias were lymphoid and were clonal and retransplantable. Overall, these findings indicate that CBF mutations cooperate with genetic alterations that accelerate G1to induce acute leukemia.

Original languageEnglish (US)
Pages (from-to)2232-2235
Number of pages4
JournalCancer Research
Volume62
Issue number8
StatePublished - Apr 15 2002

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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