Accelerated discovery via a whole-cell model

Jayodita C. Sanghvi, Sergio Regot Rodriguez De Mier, Silvia Carrasco, Jonathan R. Karr, Miriam V. Gutschow, Benjamin Bolival, Markus W. Covert

Research output: Contribution to journalArticle

Abstract

To test the promise of whole-cell modeling to facilitate scientific inquiry, we compared growth rates simulated in a whole-cell model with experimental measurements for all viable single-gene disruption Mycoplasma genitalium strains. Discrepancies between simulations and experiments led to predictions about kinetic parameters of specific enzymes that we subsequently validated. These findings represent, to our knowledge, the first application of whole-cell modeling to accelerate biological discovery.

Original languageEnglish (US)
Pages (from-to)1192-1195
Number of pages4
JournalNature Methods
Volume10
Issue number12
DOIs
StatePublished - Dec 2013
Externally publishedYes

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Kinetic parameters
Genes
Enzymes
Mycoplasma genitalium
Experiments
Theoretical Models
Growth

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Sanghvi, J. C., Regot Rodriguez De Mier, S., Carrasco, S., Karr, J. R., Gutschow, M. V., Bolival, B., & Covert, M. W. (2013). Accelerated discovery via a whole-cell model. Nature Methods, 10(12), 1192-1195. https://doi.org/10.1038/nmeth.2724

Accelerated discovery via a whole-cell model. / Sanghvi, Jayodita C.; Regot Rodriguez De Mier, Sergio; Carrasco, Silvia; Karr, Jonathan R.; Gutschow, Miriam V.; Bolival, Benjamin; Covert, Markus W.

In: Nature Methods, Vol. 10, No. 12, 12.2013, p. 1192-1195.

Research output: Contribution to journalArticle

Sanghvi, JC, Regot Rodriguez De Mier, S, Carrasco, S, Karr, JR, Gutschow, MV, Bolival, B & Covert, MW 2013, 'Accelerated discovery via a whole-cell model', Nature Methods, vol. 10, no. 12, pp. 1192-1195. https://doi.org/10.1038/nmeth.2724
Sanghvi JC, Regot Rodriguez De Mier S, Carrasco S, Karr JR, Gutschow MV, Bolival B et al. Accelerated discovery via a whole-cell model. Nature Methods. 2013 Dec;10(12):1192-1195. https://doi.org/10.1038/nmeth.2724
Sanghvi, Jayodita C. ; Regot Rodriguez De Mier, Sergio ; Carrasco, Silvia ; Karr, Jonathan R. ; Gutschow, Miriam V. ; Bolival, Benjamin ; Covert, Markus W. / Accelerated discovery via a whole-cell model. In: Nature Methods. 2013 ; Vol. 10, No. 12. pp. 1192-1195.
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