Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

T. Tsukamoto; T. Hikida

doi:10.1016/1010-6030(95)04268-7

Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

T. Tsukamoto, T. Hikida

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1 × 10⁶ dm³ mol^-1. In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm³ mol^-1 for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.

Original language	English (US)
Pages (from-to)	271-273
Number of pages	3
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	95
Issue number	3
DOIs	https://doi.org/10.1016/1010-6030(95)04268-7
State	Published - May 10 1996
Externally published	Yes

Keywords

Absorption spectra
Bovine serum albumin
Caffeine
Complex
Pyrene

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
General Physics and Astronomy

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10.1016/1010-6030(95)04268-7

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title = "Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions",

abstract = "The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1 × 106 dm3 mol-1. In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm3 mol-1 for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.",

keywords = "Absorption spectra, Bovine serum albumin, Caffeine, Complex, Pyrene",

author = "T. Tsukamoto and T. Hikida",

year = "1996",

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doi = "10.1016/1010-6030(95)04268-7",

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T1 - Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

AU - Tsukamoto, T.

AU - Hikida, T.

PY - 1996/5/10

Y1 - 1996/5/10

N2 - The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1 × 106 dm3 mol-1. In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm3 mol-1 for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.

AB - The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1 × 106 dm3 mol-1. In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm3 mol-1 for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.

KW - Absorption spectra

KW - Bovine serum albumin

KW - Caffeine

KW - Complex

KW - Pyrene

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DO - 10.1016/1010-6030(95)04268-7

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JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

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ER -

Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

Abstract

Keywords

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