Absence of early proinflammatory cytokine expression in experimental intracerebral hemorrhage

Adnan I. Qureshi, M. Fareed K. Suri, Geoffrey S.F. Ling, Jehanzeb Khan, Lee R. Guterman, L. Nelson Hopkins

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: We sought to analyze the regional concentrations of proinflammatory cytokines in the acute period of intracerebral hemorrhage (ICH) and to test the hypothesis that ICH is associated with the expression of proinflammatory cytokines in the acute period. Although the expression of cytokines and their role in neuronal injury and inflammation is well characterized in cerebral ischemia and head injury, no information exists regarding expression of cytokines in ICH. METHODS: We introduced ICH in eight anesthetized mongrel dogs by autologous blood injection (6 ml) under arterial pressure in the deep white matter adjacent to the left basal ganglia. Samples of arterial blood and cerebrospinal fluid were collected, and tissue extracts were prepared from different regions of the brain for immunoassay of tumor necrosis factor α, interleukin (IL)-1β, and IL-6 concentrations in animals with and without ICH. RESULTS: The tumor necrosis factor α levels (± standard error) in the cerebrospinal fluid 1 hour after ICH did not differ significantly between the ICH group and the control group (7.1 ± 1.3 pg/ml versus 10.8 ± 2.3 pg/ml, P = 0.22). Levels in the perihematoma region in the ICH group (96.6 ± 3.1 pg/ml) were not significantly different from those in the control group (93.4 ± 6.7 pg/ml, P = 0.7). IL-6 levels (± standard error) in the perihematoma region in the ICH group (116.3 ± 13.3 pg/ml) did not differ significantly from those in corresponding regions in the control group (122.3 ± 12.8 pg/ml, P = 0.7). IL-1β levels were below 5 pg/ml in serum, cerebrospinal fluid, and extracts of different brain regions. CONCLUSION: The early pathophysiology of ICH does not involve significant expression of tumor necrosis factor α either in the perihematoma region or other regions of the brain. The observation suggests that the pathophysiology of ICH in the acute period is different from both cerebral ischemia and traumatic brain injury.

Original languageEnglish (US)
Pages (from-to)416-421
Number of pages6
JournalNeurosurgery
Volume49
Issue number2
DOIs
StatePublished - Aug 18 2001

Keywords

  • Cerebrospinal fluid
  • Cytokines
  • Interleukins
  • Intracerebral hemorrhage
  • Tissue necrosis factor

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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