Absence of ABH antigens on neutrophils

R. A. Dunstan, M. B. Simpson, Michael J Borowitz

Research output: Contribution to journalArticle

Abstract

Many investigators have concluded that polymorphonuclear leucocytes (PMN) express ABH antigens in parallel to red cells (RBC). We have examined human PMN for ABH antigens using human isoantibodies and mouse monoclonal antibodies with three highly sensitive and specific two-stage assay systems: (1) fluorescence flow cytometry, (2) immunofluorescence microscopy, and (3) avidin-biotin immunoperoxidase microscopy. In all three assays the ABH antigens could not be detected on the surface of PMN. Previous reports alleging that ABH antigens occur on PMN probably represent false positive reactions due to inherent technical problems.

Original languageEnglish (US)
Pages (from-to)651-657
Number of pages7
JournalBritish Journal of Haematology
Volume60
Issue number4
StatePublished - 1985
Externally publishedYes

Fingerprint

Neutrophils
Antigens
False Positive Reactions
Isoantibodies
Avidin
Biotin
HLA Antigens
Fluorescence Microscopy
Microscopy
Flow Cytometry
Fluorescence
Monoclonal Antibodies
Research Personnel

ASJC Scopus subject areas

  • Hematology

Cite this

Dunstan, R. A., Simpson, M. B., & Borowitz, M. J. (1985). Absence of ABH antigens on neutrophils. British Journal of Haematology, 60(4), 651-657.

Absence of ABH antigens on neutrophils. / Dunstan, R. A.; Simpson, M. B.; Borowitz, Michael J.

In: British Journal of Haematology, Vol. 60, No. 4, 1985, p. 651-657.

Research output: Contribution to journalArticle

Dunstan, RA, Simpson, MB & Borowitz, MJ 1985, 'Absence of ABH antigens on neutrophils', British Journal of Haematology, vol. 60, no. 4, pp. 651-657.
Dunstan, R. A. ; Simpson, M. B. ; Borowitz, Michael J. / Absence of ABH antigens on neutrophils. In: British Journal of Haematology. 1985 ; Vol. 60, No. 4. pp. 651-657.
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