Many investigators have concluded that polymorphonuclear leucocytes (PMN) express ABH antigens in parallel to red cells (RBC). We have examined human PMN for ABH antigens using human isoantibodies and mouse monoclonal antibodies with three highly sensitive and specific two‐stage assay systems: (1) fluorescence flow cytometry, (2) immunofluorescence microscopy, and (3) avidinbiotin immunoperoxidase microscopy. In all three assays the ABH antigens could not be detected on the surface of PMN. Previous reports alleging that ABH antigens occur on PMN probably represent false positive reactions due to inherent technical problems.
|Original language||English (US)|
|Number of pages||7|
|Journal||British journal of haematology|
|State||Published - Aug 1985|
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