Microplasmodia of Physarum polycephalum strain M3C were exposed to 60 Hz continuous wave electric fields of 0.7 and 70 V/m for more than 13 weeks and to 45 Hz CW fields of the same strengths for nearly 7 weeks. At a particular field strength, duplicate subcultures of sham-exposed and field-exposed microplasmodia were independently maintained and measured to allow comparison of variation between subcultures within an exposure treatment group. Microplasmodial oxygen uptake rates were measured using a Clark-type oxygen electrode system, and respiratory rates were expressed on a unit protein basis. Few consistent respiratory rate differences between sham- and field-exposed subcultures were detected at either 0.7 or 70 V/m. These findings are in contrast to earlier reports of significant microplasmodial respiratory rate depression in response to 45 or 75 Hz, combined 0.7V.m, 2.0 G electric and magnetic fields and in response to 75 Hz, 0.7 V.m electric field exposure. Growth rate data are also presented and similarly fail to show an exposure treatment effect.
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging