Absence of μ opioid receptor mRNA expression in astrocytes and microglia of rat spinal cord

Sheng Chin Kao, Xiuli Zhao, Chun Yi Lee, Fidelis E. Atianjoh, Estelle B Gauda, Myron Yaster, Yuan Xiang Tao

Research output: Contribution to journalArticle

Abstract

Cumulating evidence has demonstrated that μ opioid receptor (MOR) agonists promote spinal glial activation, lead to synthesis and release of proinflammatory cytokines and chemokines, and contribute to opioid-induced hyperalgesia and development of opioid tolerance and dependence. However, whether these MOR agonists directly or indirectly act on spinal cord astrocytes and microglial cells in vivo is unclear. In the present study, by combining the techniques of in-situ hybridization of MOR mRNA with immunohistochemistry of glial fibrillary acidic protein (GFAP; an astrocyte marker) and Iba1 (a microglial marker), we examined expression and distribution of GFAP, Iba1, and MOR mRNA in the spinal cord of rats under chronic morphine tolerance conditions. Intrathecal injections of morphine twice daily for 7 days reduced morphine analgesic effect and increased both GFAP and Iba1 immunostaining densities in the spinal cord. Surprisingly, neither GFAP nor Iba1 colocalized with MOR mRNA in spinal cord cells. Our findings indicate that MOR expression is absent from spinal cord astrocytes and microglia, suggesting that these cell types are indirectly activated by MOR agonists under chronic opioid tolerance conditions.

Original languageEnglish (US)
Pages (from-to)378-384
Number of pages7
JournalNeuroReport
Volume23
Issue number6
DOIs
StatePublished - Apr 18 2012

Fingerprint

Microglia
Opioid Receptors
Astrocytes
Spinal Cord
Messenger RNA
Morphine
Opioid Analgesics
Spinal Injections
Glial Fibrillary Acidic Protein
Hyperalgesia
Chemokines
Neuroglia
In Situ Hybridization
Analgesics
Immunohistochemistry
Cytokines

Keywords

  • μ opioid receptor
  • astrocyte
  • expression
  • microglial cells
  • morphine tolerance
  • spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Absence of μ opioid receptor mRNA expression in astrocytes and microglia of rat spinal cord. / Kao, Sheng Chin; Zhao, Xiuli; Lee, Chun Yi; Atianjoh, Fidelis E.; Gauda, Estelle B; Yaster, Myron; Tao, Yuan Xiang.

In: NeuroReport, Vol. 23, No. 6, 18.04.2012, p. 378-384.

Research output: Contribution to journalArticle

Kao, Sheng Chin ; Zhao, Xiuli ; Lee, Chun Yi ; Atianjoh, Fidelis E. ; Gauda, Estelle B ; Yaster, Myron ; Tao, Yuan Xiang. / Absence of μ opioid receptor mRNA expression in astrocytes and microglia of rat spinal cord. In: NeuroReport. 2012 ; Vol. 23, No. 6. pp. 378-384.
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