Abnormalities in the p34(cdc2)-related PITSLRE protein kinase gene complex (CDC2L) on chromosome band 1p36 in melanoma

Mark A. Nelson, Maria E. Ariza, Jin Ming Yang, Floyd H. Thompson, Raymond Taetle, Jeffrey M. Trent, Julie Wymer, Kathy Massey-Brown, Marianne Broome-Powell, John Easton, Jill M. Lahti, Vincent J. Kidd

Research output: Contribution to journalArticle

Abstract

The two genes encoding the PITSLRE protein kinase isoforms, CDC2L1 and CDC2L2, are localized to human chromosome band 1p36. The PITSLRE protein kinases are a part of the p34(cdc2) supergene family. Several protein products of the CDC2L locus may be effector(s) in apoptotic signaling. The larger PITSLRE p110 isoforms appear to regulate some aspect of RNA splicing/transcription during the cell cycle. One or more of these genes may function as tumor suppressor genes in melanoma. Using fluorescence in situ hybridization, one allele of the CDC2L gene complex on chromosome 1 was either deleted or translocated in 8 of 14 different melanoma cell lines. We also observed mutations in the 5' promoter region of the CDC2L gene in four different cell lines relative to normal melanocytes using PCR-SSCP analysis and direct DNA sequencing. Western blot analysis revealed decreased level of PITSLRE protein expression in several cell lines, as well as in four surgical malignant melanoma specimens relative to normal melanocytes. Thus, the decreased PITSLRE protein expression appears to result from deletion of the CDC2L alleles and possibly by mutations within the 5'promoter region. We propose that aberrations in the CDC2L genes may contribute to the pathogenesis or progression of melanoma.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalCancer Genetics and Cytogenetics
Volume108
Issue number2
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

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Cyclin-Dependent Kinase 2
Melanoma
Chromosomes
Genes
Melanocytes
Genetic Promoter Regions
Cell Line
Protein Kinases
Protein Isoforms
Alleles
RNA Splicing
Single-Stranded Conformational Polymorphism
Mutation
Proteins
Chromosomes, Human, Pair 1
Human Chromosomes
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
DNA Sequence Analysis
Cell Cycle

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Abnormalities in the p34(cdc2)-related PITSLRE protein kinase gene complex (CDC2L) on chromosome band 1p36 in melanoma. / Nelson, Mark A.; Ariza, Maria E.; Yang, Jin Ming; Thompson, Floyd H.; Taetle, Raymond; Trent, Jeffrey M.; Wymer, Julie; Massey-Brown, Kathy; Broome-Powell, Marianne; Easton, John; Lahti, Jill M.; Kidd, Vincent J.

In: Cancer Genetics and Cytogenetics, Vol. 108, No. 2, 15.01.1999, p. 91-99.

Research output: Contribution to journalArticle

Nelson, MA, Ariza, ME, Yang, JM, Thompson, FH, Taetle, R, Trent, JM, Wymer, J, Massey-Brown, K, Broome-Powell, M, Easton, J, Lahti, JM & Kidd, VJ 1999, 'Abnormalities in the p34(cdc2)-related PITSLRE protein kinase gene complex (CDC2L) on chromosome band 1p36 in melanoma', Cancer Genetics and Cytogenetics, vol. 108, no. 2, pp. 91-99. https://doi.org/10.1016/S0165-4608(98)00122-8
Nelson, Mark A. ; Ariza, Maria E. ; Yang, Jin Ming ; Thompson, Floyd H. ; Taetle, Raymond ; Trent, Jeffrey M. ; Wymer, Julie ; Massey-Brown, Kathy ; Broome-Powell, Marianne ; Easton, John ; Lahti, Jill M. ; Kidd, Vincent J. / Abnormalities in the p34(cdc2)-related PITSLRE protein kinase gene complex (CDC2L) on chromosome band 1p36 in melanoma. In: Cancer Genetics and Cytogenetics. 1999 ; Vol. 108, No. 2. pp. 91-99.
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abstract = "The two genes encoding the PITSLRE protein kinase isoforms, CDC2L1 and CDC2L2, are localized to human chromosome band 1p36. The PITSLRE protein kinases are a part of the p34(cdc2) supergene family. Several protein products of the CDC2L locus may be effector(s) in apoptotic signaling. The larger PITSLRE p110 isoforms appear to regulate some aspect of RNA splicing/transcription during the cell cycle. One or more of these genes may function as tumor suppressor genes in melanoma. Using fluorescence in situ hybridization, one allele of the CDC2L gene complex on chromosome 1 was either deleted or translocated in 8 of 14 different melanoma cell lines. We also observed mutations in the 5' promoter region of the CDC2L gene in four different cell lines relative to normal melanocytes using PCR-SSCP analysis and direct DNA sequencing. Western blot analysis revealed decreased level of PITSLRE protein expression in several cell lines, as well as in four surgical malignant melanoma specimens relative to normal melanocytes. Thus, the decreased PITSLRE protein expression appears to result from deletion of the CDC2L alleles and possibly by mutations within the 5'promoter region. We propose that aberrations in the CDC2L genes may contribute to the pathogenesis or progression of melanoma.",
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AU - Thompson, Floyd H.

AU - Taetle, Raymond

AU - Trent, Jeffrey M.

AU - Wymer, Julie

AU - Massey-Brown, Kathy

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