Aberrant silencing of the endocrine peptide gene tachykinin-1 in gastric cancer

Stefan David, Takatsugu Kan, Yulan Cheng, Rachana Agarwal, Zhe Jin, Yuriko Mori

Research output: Contribution to journalArticle

Abstract

Tachykinin-1 (TAC1) is the precursor protein for neuroendocrine peptides, including substance P, and is centrally involved in gastric secretion, motility, mucosal immunity, and cell proliferation. Here we report aberrant silencing of TAC1 in gastric cancer (GC) by promoter hypermethylation. TAC1 methylation and mRNA expression in 47 primary GCs and 41 noncancerous gastric mucosae (NLs) were analyzed by utilizing real-time quantitative PCR-based assays. TAC1 methylation was more prevalent in GCs than in NLs: 21 (45%) of 47 GCs versus 6 (15%) of 41 NLs (p < 0.01). Microsatellite instability was also associated with TAC1 methylation in GCs. There was no significant association between TAC1 methylation and age, gender, stage, histological differentiation, or the presence of Helicobacter pylori. TAC1 mRNA was markedly downregulated in GCs relative to NLs. 5-Aza-2′-deoxycytidine-induced demethylation of the TAC1 promoter resulted in TAC1 mRNA upregulation. Further studies are indicated to elucidate the functional involvement of TAC1 in gastric carcinogenesis.

Original languageEnglish (US)
Pages (from-to)605-609
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number3
DOIs
StatePublished - Jan 16 2009

Keywords

  • Gastric cancer
  • MSI-H
  • Microsatellite instability
  • Promoter methylation
  • Real-time quantitative MSP
  • Substance P
  • Tachykinin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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