Aberrant DNA methylation of the transcription factor C/EBPα in acute myelogenous leukemia

Edgar Jost, Nicole do Ó, Stefan Wilop, James G. Herman, Rainhardt Osieka, Oliver Galm

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the methylation status of the CCAAT/enhancer binding protein alpha (C/EBPα) promoter region near the transcription start site in acute myelogenous leukemia (AML). In hematopoietic tumor cell lines, CpG island hypermethylation of the proximal C/EBPα promoter region was associated with transcriptional silencing, and treatment with the demethylating agent 5-aza-2′-deoxycytidine resulted in C/EBPα reexpression and promoter demethylation. Aberrant methylation of the C/EBPα promoter region occurred in 10/80 diagnostic AML samples, and there was an inverse correlation between aberrant methylation of C/EBPα and the negative cell cycle regulator p15. Our results provide further evidence for epigenetic dysregulation of C/EBPα in AML.

Original languageEnglish (US)
Pages (from-to)443-449
Number of pages7
JournalLeukemia Research
Volume33
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Acute myelogenous leukemia
  • C/EBP
  • Cell cycle
  • DNA methylation
  • Epigenetics
  • Transcriptional silencing

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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