ABCA1 reducing cellular arsenic accumulation in mammalian cells

X. H. Tan; C. H. Di; Y. F. Cao; L. Yang; L. L. Xian; J. Huang

doi:10.1201/b12522-85

ABCA1 reducing cellular arsenic accumulation in mammalian cells

X. H. Tan, C. H. Di, Y. F. Cao, L. Yang, L. L. Xian, J. Huang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Arsenic trioxide has been used as a therapeutic agent for many diseases and has good clinical efficacy. However, arsenic resistance in many cells limits its clinical application, and the mechanism is not fully determined. To further understand the mechanism of arsenic resistance, we constructed human arsenic-resistant ECV-304 cells (AsRE) and identified the arsenic resistant related gene ABCA1, which belonged to ATP-binding cassette subfamily in the previous study. In this study, we found that when ABCA1 expression was silenced, the AsRE cells lost their arsenic tolerance, and arsenic accumulation was greater than that of the parental ABCA1-bearing counterparts. Conversely, overexpression of ABCA1 in Hela cells decreased arsenic accumulation, and the cells were more resistant to As(III) than the control that was transfected with empty vector. Our results suggest a novel role for ABCA1 in the development of mammalian arsenic resistance.

Original language	English (US)
Title of host publication	Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress
Subtitle of host publication	Arsenic in the Environment
Publisher	Taylor and Francis - Balkema
Pages	215-217
Number of pages	3
ISBN (Print)	9780415637633
DOIs	https://doi.org/10.1201/b12522-85
State	Published - 2012
Externally published	Yes
Event	4th International Congress on Arsenic in the Environment, As 2012 - Cairns, QLD, Australia Duration: Jul 22 2012 → Jul 27 2012

Publication series

Name	Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment

Other

Other	4th International Congress on Arsenic in the Environment, As 2012
Country	Australia
City	Cairns, QLD
Period	7/22/12 → 7/27/12

ASJC Scopus subject areas

Environmental Chemistry
Pollution

Access to Document

10.1201/b12522-85

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Tan, X. H., Di, C. H., Cao, Y. F., Yang, L., Xian, L. L., & Huang, J. (2012). ABCA1 reducing cellular arsenic accumulation in mammalian cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment (pp. 215-217). (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment). Taylor and Francis - Balkema. https://doi.org/10.1201/b12522-85

ABCA1 reducing cellular arsenic accumulation in mammalian cells. / Tan, X. H.; Di, C. H.; Cao, Y. F.; Yang, L.; Xian, L. L.; Huang, J.

Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema, 2012. p. 215-217 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tan, XH, Di, CH, Cao, YF, Yang, L, Xian, LL & Huang, J 2012, ABCA1 reducing cellular arsenic accumulation in mammalian cells. in Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment, Taylor and Francis - Balkema, pp. 215-217, 4th International Congress on Arsenic in the Environment, As 2012, Cairns, QLD, Australia, 7/22/12. https://doi.org/10.1201/b12522-85

Tan XH, Di CH, Cao YF, Yang L, Xian LL, Huang J. ABCA1 reducing cellular arsenic accumulation in mammalian cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema. 2012. p. 215-217. (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment). https://doi.org/10.1201/b12522-85

Tan, X. H. ; Di, C. H. ; Cao, Y. F. ; Yang, L. ; Xian, L. L. ; Huang, J. / ABCA1 reducing cellular arsenic accumulation in mammalian cells. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Taylor and Francis - Balkema, 2012. pp. 215-217 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).

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