Ab binding alters gene expression in Cryptococcus neoformans and directly modulates fungal metabolism

Erin E. McClelland, André M. Nicola, Rafael Prados-Rosales, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

Abs facilitate humoral immunity via the classical mechanisms of opsonization, complement activation, Ab-dependent cellular cytotoxicity, and toxin/viral neutralization. There is also evidence that some Abs mediate direct antimicrobial effects. For example, Ab binding to the polysaccharide capsule of the human pathogenic fungus Cryptococcus neoformans promotes opsonization but also inhibits polysaccharide release and biofilm formation. To investigate whether Ab binding affects C. neoformans directly, we analyzed fungal gene expression after binding of protective and nonprotective mAbs. The 2 IgM Abs and 1 IgG1 Ab tested each induced different changes in gene expression. The protective IgG1 mAb upregulated genes encoding proteins involved in fatty acid synthesis, the protective IgM mAb downregulated genes encoding proteins required for protein translation, and the nonprotective IgM mAb had modest effects on gene expression. Differences in gene expression correlated with mAb binding to different locations of the capsule. Of the 3 Abs tested, the protective IgG1 mAb bound to C. neoformans closest to the cell wall, produced specific differences in the pattern of phosphorylated proteins, caused changes in lipid metabolism, and resulted in increased susceptibility to the antifungal drug amphotericin B. These results suggest what we believe to be a new mode of action for Ab-mediated immunity and raise the possibility that immunoglobulins mediate cross talk between microbes and hosts through their effects on microbial metabolism.

Original languageEnglish (US)
Pages (from-to)1355-1361
Number of pages7
JournalJournal of Clinical Investigation
Volume120
Issue number4
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

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Cryptococcus neoformans
Immunoglobulin M
Gene Expression
Immunoglobulin G
Capsules
Polysaccharides
Fungal Genes
Proteins
Complement Activation
Protein Biosynthesis
Amphotericin B
Biofilms
Humoral Immunity
Lipid Metabolism
Cell Wall
Immunoglobulins
Immunity
Fungi
Fatty Acids
Down-Regulation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ab binding alters gene expression in Cryptococcus neoformans and directly modulates fungal metabolism. / McClelland, Erin E.; Nicola, André M.; Prados-Rosales, Rafael; Casadevall, Arturo.

In: Journal of Clinical Investigation, Vol. 120, No. 4, 01.04.2010, p. 1355-1361.

Research output: Contribution to journalArticle

McClelland, Erin E. ; Nicola, André M. ; Prados-Rosales, Rafael ; Casadevall, Arturo. / Ab binding alters gene expression in Cryptococcus neoformans and directly modulates fungal metabolism. In: Journal of Clinical Investigation. 2010 ; Vol. 120, No. 4. pp. 1355-1361.
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