A whole-genome assembly of Drosophila

Eugene W. Myers, Granger G. Sutton, Art L. Delcher, Ian M. Dew, Dan P. Fasulo, Michael J. Flanigan, Saul A. Kravitz, Clark M. Mobarry, Knut H.J. Reinert, Karin A. Remington, Eric L. Anson, Randall A. Bolanos, Hui Hsien Chou, Catherine M. Jordan, Aaron L. Halpern, Stefano Lonardi, Ellen M. Beasley, Rhonda C. Brandon, Lin Chen, Patrick J. DunnZhongwu Lai, Yong Liang, Deborah R. Nusskern, Ming Zhan, Qing Zhang, Xiangqun Zheng, Gerald M. Rubin, Mark D. Adams, J. Craig Venter

Research output: Contribution to journalReview article

Abstract

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9-megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

Original languageEnglish (US)
Pages (from-to)2196-2204
Number of pages9
JournalScience
Volume287
Issue number5461
DOIs
StatePublished - Mar 24 2000

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    Myers, E. W., Sutton, G. G., Delcher, A. L., Dew, I. M., Fasulo, D. P., Flanigan, M. J., Kravitz, S. A., Mobarry, C. M., Reinert, K. H. J., Remington, K. A., Anson, E. L., Bolanos, R. A., Chou, H. H., Jordan, C. M., Halpern, A. L., Lonardi, S., Beasley, E. M., Brandon, R. C., Chen, L., ... Venter, J. C. (2000). A whole-genome assembly of Drosophila. Science, 287(5461), 2196-2204. https://doi.org/10.1126/science.287.5461.2196