A whole blood bactericidal assay for tuberculosis

R. S. Wallis; M. Palaci; S. Vinhas; A. G. Hise; F. C. Ribeiro; K. Landen; S. H. Cheon; H. Y. Song; M. Phillips; R. Dietze; J. J. Elner

doi:10.1086/319679

A whole blood bactericidal assay for tuberculosis

R. S. Wallis, M. Palaci, S. Vinhas, A. G. Hise, F. C. Ribeiro, K. Landen, S. H. Cheon, H. Y. Song, M. Phillips, R. Dietze, J. J. Elner

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87 Scopus citations

Abstract

The bactericidal activity of orally administered antituberculosis (anti-TB) drugs was determined in a whole blood culture model of intracellular infection in which microbial killing reflects the combined effects of drug and immune mechanisms. Rifampin (Rif) was the most active compound studied and reduced the number of viable bacilli by >4 logs. Isoniazid (INH), 2 quinolones, and pyrazinamide (PZA) showed intermediate levels of activity. Ethambutol exerted only a bacteristatic effect; amoxicillin/clavulanate was inactive. The combination of INH-Rif-PZA showed strong activity against 11 drug-sensitive isolates (mean, -3.8 log) but no activity against 12 multidrug-resistant (MDR) strains. The combination of levofloxacin-PZA-ethambutol had intermediate bactericidal activity against MDR isolates (mean, -1.2 log) but failed to equal that of INH-Rif-PZA against sensitive isolates (P <.001). The whole blood BACTEC method (Becton Dickinson) may be useful for the early clinical evaluation of new anti-TB drugs and in the management of individual patients.

Original language	English (US)
Pages (from-to)	1300-1303
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	183
Issue number	8
DOIs	https://doi.org/10.1086/319679
State	Published - Apr 15 2001
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Immunology

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10.1086/319679

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title = "A whole blood bactericidal assay for tuberculosis",

abstract = "The bactericidal activity of orally administered antituberculosis (anti-TB) drugs was determined in a whole blood culture model of intracellular infection in which microbial killing reflects the combined effects of drug and immune mechanisms. Rifampin (Rif) was the most active compound studied and reduced the number of viable bacilli by >4 logs. Isoniazid (INH), 2 quinolones, and pyrazinamide (PZA) showed intermediate levels of activity. Ethambutol exerted only a bacteristatic effect; amoxicillin/clavulanate was inactive. The combination of INH-Rif-PZA showed strong activity against 11 drug-sensitive isolates (mean, -3.8 log) but no activity against 12 multidrug-resistant (MDR) strains. The combination of levofloxacin-PZA-ethambutol had intermediate bactericidal activity against MDR isolates (mean, -1.2 log) but failed to equal that of INH-Rif-PZA against sensitive isolates (P <.001). The whole blood BACTEC method (Becton Dickinson) may be useful for the early clinical evaluation of new anti-TB drugs and in the management of individual patients.",

author = "Wallis, {R. S.} and M. Palaci and S. Vinhas and Hise, {A. G.} and Ribeiro, {F. C.} and K. Landen and Cheon, {S. H.} and Song, {H. Y.} and M. Phillips and R. Dietze and Elner, {J. J.}",

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AU - Wallis, R. S.

AU - Palaci, M.

AU - Vinhas, S.

AU - Hise, A. G.

AU - Ribeiro, F. C.

AU - Landen, K.

AU - Cheon, S. H.

AU - Song, H. Y.

AU - Phillips, M.

AU - Dietze, R.

AU - Elner, J. J.

PY - 2001/4/15

Y1 - 2001/4/15

N2 - The bactericidal activity of orally administered antituberculosis (anti-TB) drugs was determined in a whole blood culture model of intracellular infection in which microbial killing reflects the combined effects of drug and immune mechanisms. Rifampin (Rif) was the most active compound studied and reduced the number of viable bacilli by >4 logs. Isoniazid (INH), 2 quinolones, and pyrazinamide (PZA) showed intermediate levels of activity. Ethambutol exerted only a bacteristatic effect; amoxicillin/clavulanate was inactive. The combination of INH-Rif-PZA showed strong activity against 11 drug-sensitive isolates (mean, -3.8 log) but no activity against 12 multidrug-resistant (MDR) strains. The combination of levofloxacin-PZA-ethambutol had intermediate bactericidal activity against MDR isolates (mean, -1.2 log) but failed to equal that of INH-Rif-PZA against sensitive isolates (P <.001). The whole blood BACTEC method (Becton Dickinson) may be useful for the early clinical evaluation of new anti-TB drugs and in the management of individual patients.

AB - The bactericidal activity of orally administered antituberculosis (anti-TB) drugs was determined in a whole blood culture model of intracellular infection in which microbial killing reflects the combined effects of drug and immune mechanisms. Rifampin (Rif) was the most active compound studied and reduced the number of viable bacilli by >4 logs. Isoniazid (INH), 2 quinolones, and pyrazinamide (PZA) showed intermediate levels of activity. Ethambutol exerted only a bacteristatic effect; amoxicillin/clavulanate was inactive. The combination of INH-Rif-PZA showed strong activity against 11 drug-sensitive isolates (mean, -3.8 log) but no activity against 12 multidrug-resistant (MDR) strains. The combination of levofloxacin-PZA-ethambutol had intermediate bactericidal activity against MDR isolates (mean, -1.2 log) but failed to equal that of INH-Rif-PZA against sensitive isolates (P <.001). The whole blood BACTEC method (Becton Dickinson) may be useful for the early clinical evaluation of new anti-TB drugs and in the management of individual patients.

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A whole blood bactericidal assay for tuberculosis

Abstract

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