Abstract
The effect of diet on reproduction is well documented in a large number of organisms; however, much remains to be learned about the molecular mechanisms underlying this connection. The Drosophila ovary has a well described, fast and largely reversible response to diet. Ovarian stem cells and their progeny proliferate and grow faster on a yeast-rich diet than on a yeast-free (poor) diet, and death of early germline cysts, degeneration of early vitellogenic follicles and partial block in ovulation further contribute to the ∼60-fold decrease in egg laying observed on a poor diet. Multiple diet-dependent factors, including insulin-like peptides, the steroid ecdysone, the nutrient sensor Target of Rapamycin, AMP-dependent kinase, and adipocyte factors mediate this complex response. Here, we describe the results of a visual screen using a collection of green fluorescent protein (GFP) protein trap lines to identify additional factors potentially involved in this response. In each GFP protein trap line, an artificial GFP exon is fused in frame to an endogenous protein, such that the GFP fusion pattern parallels the levels and subcellular localization of the corresponding native protein. We identified 53 GFP-tagged proteins that exhibit changes in levels and/or subcellular localization in the ovary at 12–16 hours after switching females from rich to poor diets, suggesting them as potential candidates for future functional studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13-21 |
| Number of pages | 9 |
| Journal | Gene Expression Patterns |
| Volume | 23-24 |
| DOIs | |
| State | Published - Jan 1 2017 |
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Keywords
- Diet
- Drosophila
- Germline stem cells
- GFP protein trap line
- Oogenesis
- Ovary
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Developmental Biology
Cite this
A visual screen for diet-regulated proteins in the Drosophila ovary using GFP protein trap lines. / Hsu, Hwei Jan; Barbosa, Daniela Drummond.
In: Gene Expression Patterns, Vol. 23-24, 01.01.2017, p. 13-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A visual screen for diet-regulated proteins in the Drosophila ovary using GFP protein trap lines
AU - Hsu, Hwei Jan
AU - Barbosa, Daniela Drummond
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The effect of diet on reproduction is well documented in a large number of organisms; however, much remains to be learned about the molecular mechanisms underlying this connection. The Drosophila ovary has a well described, fast and largely reversible response to diet. Ovarian stem cells and their progeny proliferate and grow faster on a yeast-rich diet than on a yeast-free (poor) diet, and death of early germline cysts, degeneration of early vitellogenic follicles and partial block in ovulation further contribute to the ∼60-fold decrease in egg laying observed on a poor diet. Multiple diet-dependent factors, including insulin-like peptides, the steroid ecdysone, the nutrient sensor Target of Rapamycin, AMP-dependent kinase, and adipocyte factors mediate this complex response. Here, we describe the results of a visual screen using a collection of green fluorescent protein (GFP) protein trap lines to identify additional factors potentially involved in this response. In each GFP protein trap line, an artificial GFP exon is fused in frame to an endogenous protein, such that the GFP fusion pattern parallels the levels and subcellular localization of the corresponding native protein. We identified 53 GFP-tagged proteins that exhibit changes in levels and/or subcellular localization in the ovary at 12–16 hours after switching females from rich to poor diets, suggesting them as potential candidates for future functional studies.
AB - The effect of diet on reproduction is well documented in a large number of organisms; however, much remains to be learned about the molecular mechanisms underlying this connection. The Drosophila ovary has a well described, fast and largely reversible response to diet. Ovarian stem cells and their progeny proliferate and grow faster on a yeast-rich diet than on a yeast-free (poor) diet, and death of early germline cysts, degeneration of early vitellogenic follicles and partial block in ovulation further contribute to the ∼60-fold decrease in egg laying observed on a poor diet. Multiple diet-dependent factors, including insulin-like peptides, the steroid ecdysone, the nutrient sensor Target of Rapamycin, AMP-dependent kinase, and adipocyte factors mediate this complex response. Here, we describe the results of a visual screen using a collection of green fluorescent protein (GFP) protein trap lines to identify additional factors potentially involved in this response. In each GFP protein trap line, an artificial GFP exon is fused in frame to an endogenous protein, such that the GFP fusion pattern parallels the levels and subcellular localization of the corresponding native protein. We identified 53 GFP-tagged proteins that exhibit changes in levels and/or subcellular localization in the ovary at 12–16 hours after switching females from rich to poor diets, suggesting them as potential candidates for future functional studies.
KW - Diet
KW - Drosophila
KW - Germline stem cells
KW - GFP protein trap line
KW - Oogenesis
KW - Ovary
UR - http://www.scopus.com/inward/record.url?scp=85009841241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009841241&partnerID=8YFLogxK
U2 - 10.1016/j.gep.2017.01.001
DO - 10.1016/j.gep.2017.01.001
M3 - Article
VL - 23-24
SP - 13
EP - 21
JO - Gene Expression Patterns
JF - Gene Expression Patterns
SN - 1567-133X
ER -