A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection

Wataru Akahata, Zhi Yong Yang, Hanne Andersen, Siyang Sun, Heather A. Holdaway, Wing Pui Kong, Mark G. Lewis, Stephen Higgs, Michael G. Rossmann, Srinivas Rao, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

Chikungunya virus (CHIKV) has infected millions of people in Africa, Europe and Asia since this alphavirus reemerged from Kenya in 2004. The severity of the disease and the spread of this epidemic virus present a serious public health threat in the absence of vaccines or antiviral therapies. Here, we describe a new vaccine that protects against CHIKV infection of nonhuman primates. We show that selective expression of viral structural proteins gives rise to virus-like particles (VLPs) in vitro that resemble replication-competent alphaviruses. Immunization with these VLPs elicited neutralizing antibodies against envelope proteins from alternative CHIKV strains. Monkeys immunized with VLPs produced high-titer neutralizing antibodies that protected against viremia after high-dose challenge. We transferred these antibodies into immunodeficient mice, where they protected against subsequent lethal CHIKV challenge, indicating a humoral mechanism of protection. Immunization with alphavirus VLP vaccines represents a strategy to contain the spread of CHIKV and related pathogenic viruses in humans.

Original languageEnglish (US)
Pages (from-to)334-338
Number of pages5
JournalNature medicine
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Akahata, W., Yang, Z. Y., Andersen, H., Sun, S., Holdaway, H. A., Kong, W. P., Lewis, M. G., Higgs, S., Rossmann, M. G., Rao, S., & Nabel, G. J. (2010). A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection. Nature medicine, 16(3), 334-338. https://doi.org/10.1038/nm.2105