A validated new method for nevirapine quantitation in human plasma via high-performance liquid chromatography

Courtney F. Silverthorn, Teresa L. Parsons

Research output: Contribution to journalArticle

Abstract

A fully validated and clinically relevant assay was developed for the assessment of nevirapine concentrations in neonate blood plasma samples. Solid-phase extraction with an acid-base wash series was used to prepare subject samples for analysis. Samples were separated by high performance liquid chromatography and detected at 280 nm on a C8 reverse-phase column in an isocratic mobile phase. The retention times of nevirapine and its internal standard were 5.0 and 6.9 min, respectively. The method was validated by assessment of accuracy and precision (statistical values 2 > 0.996) and the average recovery was 93% (n = 18). The lower limit of quantification (relative standard deviation

Original languageEnglish (US)
Pages (from-to)23-27
Number of pages5
JournalBiomedical Chromatography
Volume20
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Plasma (human)
Nevirapine
High performance liquid chromatography
High Pressure Liquid Chromatography
Solid Phase Extraction
Assays
Blood
Plasmas
Recovery
Acids

Keywords

  • HIV drug analysis
  • Nevirapine
  • Non-nucleoside reverse transcriptase inhibitor
  • Reverse-phase liquid chromatography
  • Solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

A validated new method for nevirapine quantitation in human plasma via high-performance liquid chromatography. / Silverthorn, Courtney F.; Parsons, Teresa L.

In: Biomedical Chromatography, Vol. 20, No. 1, 01.2006, p. 23-27.

Research output: Contribution to journalArticle

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