A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk

Robin E. Choo, Lauren M. Jansson, Karl Scheidweiler, Marilyn A. Huestis

Research output: Contribution to journalArticlepeer-review

Abstract

This manuscript details a validated liquid chromatography-atmospheric pressure chemical ionizationtandem mass spectrometry (LC-APCI-MS-MS) method for the quantification of methadone and its metabolites 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in 0.5 mL human breast milk. Limits of detection were 5 ng/mL for methadone and EDDP, and 10 ng/mL for EMDP. Linearity ranged from 10 to 500 ng/mL for all analytes. Breast milk is a complex biological fluid, necessitating several specimen preparation steps to separate methadone and metabolites from the lipophilic matrix. Recoveries were 66-97% following protein precipitation and solid-phase extraction with minimal matrix effect. Acceptable accuracy (89-101%) and precision (15-20% RSD) were achieved for all analytes. This is the first LC-APCI-MS-MS method for the sensitive and specific detection of methadone, EDDP, and EMDP in human breast milk. The method proved suitable for quantification of methadone and metabolites in breast milk of methadone-maintained opiate-dependent women.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalJournal of analytical toxicology
Volume31
Issue number5
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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