A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk

Robin E. Choo; Lauren M. Jansson; Karl Scheidweiler; Marilyn A. Huestis

doi:10.1093/jat/31.5.265

A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk

Robin E. Choo, Lauren M. Jansson, Karl Scheidweiler, Marilyn A. Huestis

School of Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

This manuscript details a validated liquid chromatography-atmospheric pressure chemical ionizationtandem mass spectrometry (LC-APCI-MS-MS) method for the quantification of methadone and its metabolites 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in 0.5 mL human breast milk. Limits of detection were 5 ng/mL for methadone and EDDP, and 10 ng/mL for EMDP. Linearity ranged from 10 to 500 ng/mL for all analytes. Breast milk is a complex biological fluid, necessitating several specimen preparation steps to separate methadone and metabolites from the lipophilic matrix. Recoveries were 66-97% following protein precipitation and solid-phase extraction with minimal matrix effect. Acceptable accuracy (89-101%) and precision (15-20% RSD) were achieved for all analytes. This is the first LC-APCI-MS-MS method for the sensitive and specific detection of methadone, EDDP, and EMDP in human breast milk. The method proved suitable for quantification of methadone and metabolites in breast milk of methadone-maintained opiate-dependent women.

Original language	English (US)
Pages (from-to)	265-269
Number of pages	5
Journal	Journal of analytical toxicology
Volume	31
Issue number	5
DOIs	https://doi.org/10.1093/jat/31.5.265
State	Published - Jun 2007

ASJC Scopus subject areas

Analytical Chemistry
Environmental Chemistry
Toxicology
Health, Toxicology and Mutagenesis
Chemical Health and Safety

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Choo, R. E., Jansson, L. M., Scheidweiler, K., & Huestis, M. A. (2007). A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk. Journal of analytical toxicology, 31(5), 265-269. https://doi.org/10.1093/jat/31.5.265

A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk. / Choo, Robin E.; Jansson, Lauren M.; Scheidweiler, Karl et al.
In: Journal of analytical toxicology, Vol. 31, No. 5, 06.2007, p. 265-269.

Research output: Contribution to journal › Article › peer-review

Choo, RE, Jansson, LM, Scheidweiler, K & Huestis, MA 2007, 'A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk', Journal of analytical toxicology, vol. 31, no. 5, pp. 265-269. https://doi.org/10.1093/jat/31.5.265

Choo RE, Jansson LM, Scheidweiler K, Huestis MA. A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk. Journal of analytical toxicology. 2007 Jun;31(5):265-269. doi: 10.1093/jat/31.5.265

Choo, Robin E. ; Jansson, Lauren M. ; Scheidweiler, Karl et al. / A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk. In: Journal of analytical toxicology. 2007 ; Vol. 31, No. 5. pp. 265-269.

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title = "A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk",

abstract = "This manuscript details a validated liquid chromatography-atmospheric pressure chemical ionizationtandem mass spectrometry (LC-APCI-MS-MS) method for the quantification of methadone and its metabolites 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in 0.5 mL human breast milk. Limits of detection were 5 ng/mL for methadone and EDDP, and 10 ng/mL for EMDP. Linearity ranged from 10 to 500 ng/mL for all analytes. Breast milk is a complex biological fluid, necessitating several specimen preparation steps to separate methadone and metabolites from the lipophilic matrix. Recoveries were 66-97% following protein precipitation and solid-phase extraction with minimal matrix effect. Acceptable accuracy (89-101%) and precision (15-20% RSD) were achieved for all analytes. This is the first LC-APCI-MS-MS method for the sensitive and specific detection of methadone, EDDP, and EMDP in human breast milk. The method proved suitable for quantification of methadone and metabolites in breast milk of methadone-maintained opiate-dependent women.",

author = "Choo, {Robin E.} and Jansson, {Lauren M.} and Karl Scheidweiler and Huestis, {Marilyn A.}",

note = "Funding Information: This study was supported by Grant K08 DA00495 from the National Institute on Health and the National Institute on Drug Abuse Extramural Research Grant awarded to Dr. Lauren Jansson and National Institute on Drug Abuse intramural funds. We would like to acknowledge the staff at the Center for Addiction and Pregnancy and postpartum wards of the Johns Hopkins Bayview Medical Center for assistance during the course of the study.",

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AU - Choo, Robin E.

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AB - This manuscript details a validated liquid chromatography-atmospheric pressure chemical ionizationtandem mass spectrometry (LC-APCI-MS-MS) method for the quantification of methadone and its metabolites 2-ethylidene-1,5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in 0.5 mL human breast milk. Limits of detection were 5 ng/mL for methadone and EDDP, and 10 ng/mL for EMDP. Linearity ranged from 10 to 500 ng/mL for all analytes. Breast milk is a complex biological fluid, necessitating several specimen preparation steps to separate methadone and metabolites from the lipophilic matrix. Recoveries were 66-97% following protein precipitation and solid-phase extraction with minimal matrix effect. Acceptable accuracy (89-101%) and precision (15-20% RSD) were achieved for all analytes. This is the first LC-APCI-MS-MS method for the sensitive and specific detection of methadone, EDDP, and EMDP in human breast milk. The method proved suitable for quantification of methadone and metabolites in breast milk of methadone-maintained opiate-dependent women.

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A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk

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