@article{d757bd4bae434f8380234bca4c8f500c,
title = "A unique rabbit immunoglobulin having homocytotropic antibody activity",
abstract = "Evidence was obtained that rabbit homocytotropic antibody against dinitrophenyl-bovine γ globulin (DNP-BGG) belonged to a unique immunoglobulin other than γG, γA or γM. The skin-sensitizing activity was precipitated with a guinea pig anti-rabbit globulin serum which did not contain any of the antibodies specific for the three immunoglobulins and for light chains of immunoglobulins. The guinea pig antiserum gave a β precipitin band with a rabbit serum fraction which contained high skin sensitizing activity and the precipitin band combined 131I-labeled DNP-BGG. When rabbit antisera having the homocytotropic antibody were fractionated by chromatography on a DEAE cellulose column, gel filtration, sucrose density gradient ultracentrifugation and zone electrophoresis on agarose gel, distribution of the homocytotropic antibody paralleled those of the β globulin antibody detected by radioimmunodiffusion. Light chains o f immunoglobulins were detected in the β globulin, indicating that the protein represents an immunoglobulin. Since the physicochemical properties and biologic function of the β immunoglobulin were similar to human γE, the protein was tentatively designated rabbit γE. Guinea pig antibody against γE-induced an increased permeability of rabbit skin small vessels.",
author = "Kimishige Ishizaka and Teruko Ishizaka and Hornbrook, {Margaret M.}",
note = "Funding Information: Rabbit antibodies capable of sensitizing the skin of the homologous species for passive cutaneous anaphylaxis (PCA) were described by Onoue et a/.\[1\]a nd by Zvaifler and Becker\[2\] in 1966. The latter investigators immunized rabbits with dinitrophenyl (DNP) bovine-y-globulin (BGG) included in complete Freund's adjuvant and detected skin-sensitizing antibody in antisera obtained one week after the immunization. The antibody disappeared from the circulation after a few days or weeks and did not reappear on subsequent booster injections. The rabbit anaphylactic (homocytotropic) antibodies are similar to human yE reaginic antibodies with respect to their biologic properties. Both human and rabbit antibodies persist at passively sensitized skin sites for a long period of time and lose their sensitizing activity by heating at 56°C for 2-4 hr. More recently, Lindqvist \[3\]h as shown that footpad injection of alum-precipitated tetanus toxoid into rabbits resulted in the formation of a homocytotropic antibody, which was detectable in the serum for more than 4 months after the primary immunization. In this system, the PCA titer of the antiserum increased by booster injections and the antibody was heat-stable. Physicochemically, however, the antitetanus antibody was similar to the heat-labile antibody described by Zvaifler and Becker\[2\]. Both antibodies are electrophoretically *This work was supported by research Grant No. GB-8370 from the National Science Foundation. A part of this paper was presented before the 53rd Annual Meeting of the American Association of Immunologists held in Atlantic City, N.J., April, 1969. 515",
year = "1970",
month = jun,
doi = "10.1016/0019-2791(70)90273-9",
language = "English (US)",
volume = "7",
pages = "515--520,IN1--IN2",
journal = "Immunochemistry",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "6",
}