TY - JOUR
T1 - A U3 small nuclear ribonucleoprotein-requiring processing event in the 5′ external transcribed spacer of Xenopus precursor rRNA
AU - Mougey, Edward B.
AU - Pape, Louise K.
AU - Sollner-Webb, Barbara
PY - 1993/10
Y1 - 1993/10
N2 - A processing site has been identified within the 5′ external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5′ ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5′ ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5′ ETS processing site serves an evolutionarily selective function.
AB - A processing site has been identified within the 5′ external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5′ ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5′ ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5′ ETS processing site serves an evolutionarily selective function.
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U2 - 10.1128/MCB.13.10.5990
DO - 10.1128/MCB.13.10.5990
M3 - Article
C2 - 8413202
AN - SCOPUS:0027442923
SN - 0270-7306
VL - 13
SP - 5990
EP - 5998
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 10
ER -