A Type III restriction - Modification system in Mycoplasma mycoides subsp. capri

Mikkel A. Algire, Michael G. Montague, Sanjay Vashee, Carole Lartigue, Chuck Merryman

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction-modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and methylate the adenine. The coding region of the methyl-transferase gene contains 12 consecutive AG dinucleotide repeats that result in a translational termination at a TAA codon immediately beyond the repeat region. This strain does not have MmyCI activity. A clone was found with 10 AG repeats such that the gene is in frame, and this strain has MmyCI activity, suggesting that the expression of the MmyCI methyltransferase may be phase variable.

Original languageEnglish (US)
Article number120115
JournalOpen Biology
Issue numberOCT
StatePublished - 2012
Externally publishedYes


  • Methyltransferase
  • Mycoplasma
  • Phase variation
  • Type III

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)


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