A tunable FRET circuit for engineering fluorescent biosensors

Michael D. Allen, Jin Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

(Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.

Original languageEnglish (US)
Pages (from-to)500-502
Number of pages3
JournalAngewandte Chemie - International Edition
Volume47
Issue number3
DOIs
StatePublished - 2008

Keywords

  • Fluorescence
  • FRET (fluorescence resonant energy transfer)
  • Kinases
  • Microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Medicine(all)

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