A tunable FRET circuit for engineering fluorescent biosensors

Michael D. Allen; Jin Zhang

doi:10.1002/anie.200703493

A tunable FRET circuit for engineering fluorescent biosensors

Michael D. Allen, Jin Zhang

School of Medicine

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

(Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.

Original language	English (US)
Pages (from-to)	500-502
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	3
DOIs	https://doi.org/10.1002/anie.200703493
State	Published - 2008

Keywords

Fluorescence
FRET (fluorescence resonant energy transfer)
Kinases
Microscopy

ASJC Scopus subject areas

General Chemistry
General Medicine

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10.1002/anie.200703493

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abstract = "(Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.",

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AU - Zhang, Jin

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AB - (Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.

KW - Fluorescence

KW - FRET (fluorescence resonant energy transfer)

KW - Kinases

KW - Microscopy

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A tunable FRET circuit for engineering fluorescent biosensors

Abstract

Keywords

ASJC Scopus subject areas

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