A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms

R. F. Souza, J. Lei, J. Yin, R. Appel, T. T. Zou, X. Zhou, Suna Wang, G. Rhyu M.-, K. Cymes, O. Chan, W. S. Park, M. J. Krasna, B. D. Greenwald, J. Cottrell, J. M. Abraham, L. Simms, B. Leggett, J. Young, N. Harpaz, Stephen Meltzer

Research output: Contribution to journalArticle

Abstract

Background and Aims: Numerous gastrointestinal tumors, notably sporadic and ulcerative colitis (UC)-associated colorectal carcinomas and dysplasias, gastric cancers, and esophageal carcinomas, manifest microsatellite instability. Recently, a transforming growth factor β1 type II receptor (TGF-β1RII) mutation in a coding microsatellite was described in colorectal carcinomas showing instability. One hundred thirty-eight human neoplasms (61 UC-associated, 35 gastric, 26 esophageal, and 16 sporadic colorectal) were evaluated for this TGF-β1RII mutation. Methods: Whether instability was present at other chromosomal loci in these lesions was determined. In lesions manifesting or lacking instability, the TGF-β1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reaction amplified with 32P-labeled deoxycytidine triphosphate. Polymerase chain reaction products were electrophoresed on denaturing gels and exposed to radiographic film. Results: Three of 18 UC specimens with instability at other chromosomal loci (17%) showed TGF-β1RII poly A tract mutation, including 2 cancers and 1 dysplasia; moreover, 2% of UC specimens without instability (1 of 43) (1 cancer), 81% of unstable sporadic colorectal cancers (13 of 16), and none of the 61 stable or unstable gastric or esophageal cancers contained TGF-β1RII mutations. Conclusions: Mutational inactivation of the poly A microsatellite tract within TGF-β1RII occurs early and in a subset of unstable UC neoplasms and commonly in sporadic colorectal cancers but may be rare in unstable gastric and esophageal tumors.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalGastroenterology
Volume112
Issue number1
StatePublished - 1997
Externally publishedYes

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Growth Factor Receptors
Transforming Growth Factors
Ulcerative Colitis
Mutation
Colorectal Neoplasms
Microsatellite Repeats
Neoplasms
Stomach Neoplasms
Stomach
Polymerase Chain Reaction
Microsatellite Instability
X-Ray Film
Esophageal Neoplasms
Gels
Carcinoma

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Souza, R. F., Lei, J., Yin, J., Appel, R., Zou, T. T., Zhou, X., ... Meltzer, S. (1997). A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms. Gastroenterology, 112(1), 40-45.

A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms. / Souza, R. F.; Lei, J.; Yin, J.; Appel, R.; Zou, T. T.; Zhou, X.; Wang, Suna; Rhyu M.-, G.; Cymes, K.; Chan, O.; Park, W. S.; Krasna, M. J.; Greenwald, B. D.; Cottrell, J.; Abraham, J. M.; Simms, L.; Leggett, B.; Young, J.; Harpaz, N.; Meltzer, Stephen.

In: Gastroenterology, Vol. 112, No. 1, 1997, p. 40-45.

Research output: Contribution to journalArticle

Souza, RF, Lei, J, Yin, J, Appel, R, Zou, TT, Zhou, X, Wang, S, Rhyu M.-, G, Cymes, K, Chan, O, Park, WS, Krasna, MJ, Greenwald, BD, Cottrell, J, Abraham, JM, Simms, L, Leggett, B, Young, J, Harpaz, N & Meltzer, S 1997, 'A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms', Gastroenterology, vol. 112, no. 1, pp. 40-45.
Souza, R. F. ; Lei, J. ; Yin, J. ; Appel, R. ; Zou, T. T. ; Zhou, X. ; Wang, Suna ; Rhyu M.-, G. ; Cymes, K. ; Chan, O. ; Park, W. S. ; Krasna, M. J. ; Greenwald, B. D. ; Cottrell, J. ; Abraham, J. M. ; Simms, L. ; Leggett, B. ; Young, J. ; Harpaz, N. ; Meltzer, Stephen. / A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms. In: Gastroenterology. 1997 ; Vol. 112, No. 1. pp. 40-45.
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abstract = "Background and Aims: Numerous gastrointestinal tumors, notably sporadic and ulcerative colitis (UC)-associated colorectal carcinomas and dysplasias, gastric cancers, and esophageal carcinomas, manifest microsatellite instability. Recently, a transforming growth factor β1 type II receptor (TGF-β1RII) mutation in a coding microsatellite was described in colorectal carcinomas showing instability. One hundred thirty-eight human neoplasms (61 UC-associated, 35 gastric, 26 esophageal, and 16 sporadic colorectal) were evaluated for this TGF-β1RII mutation. Methods: Whether instability was present at other chromosomal loci in these lesions was determined. In lesions manifesting or lacking instability, the TGF-β1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reaction amplified with 32P-labeled deoxycytidine triphosphate. Polymerase chain reaction products were electrophoresed on denaturing gels and exposed to radiographic film. Results: Three of 18 UC specimens with instability at other chromosomal loci (17{\%}) showed TGF-β1RII poly A tract mutation, including 2 cancers and 1 dysplasia; moreover, 2{\%} of UC specimens without instability (1 of 43) (1 cancer), 81{\%} of unstable sporadic colorectal cancers (13 of 16), and none of the 61 stable or unstable gastric or esophageal cancers contained TGF-β1RII mutations. Conclusions: Mutational inactivation of the poly A microsatellite tract within TGF-β1RII occurs early and in a subset of unstable UC neoplasms and commonly in sporadic colorectal cancers but may be rare in unstable gastric and esophageal tumors.",
author = "Souza, {R. F.} and J. Lei and J. Yin and R. Appel and Zou, {T. T.} and X. Zhou and Suna Wang and {Rhyu M.-}, G. and K. Cymes and O. Chan and Park, {W. S.} and Krasna, {M. J.} and Greenwald, {B. D.} and J. Cottrell and Abraham, {J. M.} and L. Simms and B. Leggett and J. Young and N. Harpaz and Stephen Meltzer",
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T1 - A transforming growth factor β1 receptor type II mutation in ulcerative colitis-associated neoplasms

AU - Souza, R. F.

AU - Lei, J.

AU - Yin, J.

AU - Appel, R.

AU - Zou, T. T.

AU - Zhou, X.

AU - Wang, Suna

AU - Rhyu M.-, G.

AU - Cymes, K.

AU - Chan, O.

AU - Park, W. S.

AU - Krasna, M. J.

AU - Greenwald, B. D.

AU - Cottrell, J.

AU - Abraham, J. M.

AU - Simms, L.

AU - Leggett, B.

AU - Young, J.

AU - Harpaz, N.

AU - Meltzer, Stephen

PY - 1997

Y1 - 1997

N2 - Background and Aims: Numerous gastrointestinal tumors, notably sporadic and ulcerative colitis (UC)-associated colorectal carcinomas and dysplasias, gastric cancers, and esophageal carcinomas, manifest microsatellite instability. Recently, a transforming growth factor β1 type II receptor (TGF-β1RII) mutation in a coding microsatellite was described in colorectal carcinomas showing instability. One hundred thirty-eight human neoplasms (61 UC-associated, 35 gastric, 26 esophageal, and 16 sporadic colorectal) were evaluated for this TGF-β1RII mutation. Methods: Whether instability was present at other chromosomal loci in these lesions was determined. In lesions manifesting or lacking instability, the TGF-β1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reaction amplified with 32P-labeled deoxycytidine triphosphate. Polymerase chain reaction products were electrophoresed on denaturing gels and exposed to radiographic film. Results: Three of 18 UC specimens with instability at other chromosomal loci (17%) showed TGF-β1RII poly A tract mutation, including 2 cancers and 1 dysplasia; moreover, 2% of UC specimens without instability (1 of 43) (1 cancer), 81% of unstable sporadic colorectal cancers (13 of 16), and none of the 61 stable or unstable gastric or esophageal cancers contained TGF-β1RII mutations. Conclusions: Mutational inactivation of the poly A microsatellite tract within TGF-β1RII occurs early and in a subset of unstable UC neoplasms and commonly in sporadic colorectal cancers but may be rare in unstable gastric and esophageal tumors.

AB - Background and Aims: Numerous gastrointestinal tumors, notably sporadic and ulcerative colitis (UC)-associated colorectal carcinomas and dysplasias, gastric cancers, and esophageal carcinomas, manifest microsatellite instability. Recently, a transforming growth factor β1 type II receptor (TGF-β1RII) mutation in a coding microsatellite was described in colorectal carcinomas showing instability. One hundred thirty-eight human neoplasms (61 UC-associated, 35 gastric, 26 esophageal, and 16 sporadic colorectal) were evaluated for this TGF-β1RII mutation. Methods: Whether instability was present at other chromosomal loci in these lesions was determined. In lesions manifesting or lacking instability, the TGF-β1RII coding region polydeoxyadenine (poly A) microsatellite tract was polymerase chain reaction amplified with 32P-labeled deoxycytidine triphosphate. Polymerase chain reaction products were electrophoresed on denaturing gels and exposed to radiographic film. Results: Three of 18 UC specimens with instability at other chromosomal loci (17%) showed TGF-β1RII poly A tract mutation, including 2 cancers and 1 dysplasia; moreover, 2% of UC specimens without instability (1 of 43) (1 cancer), 81% of unstable sporadic colorectal cancers (13 of 16), and none of the 61 stable or unstable gastric or esophageal cancers contained TGF-β1RII mutations. Conclusions: Mutational inactivation of the poly A microsatellite tract within TGF-β1RII occurs early and in a subset of unstable UC neoplasms and commonly in sporadic colorectal cancers but may be rare in unstable gastric and esophageal tumors.

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