TY - JOUR
T1 - A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA gene
AU - Henderson, Sheryl
AU - Sollner-Webb, Barbara
N1 - Funding Information:
This work was supported by a grant from the National Institutes of Health (GM27720). We thank Ms. Maria Ysern for technical assistance and Ms. Sue Millionie for help in preparing the manuscript.
PY - 1986/12/26
Y1 - 1986/12/26
N2 - Sequences flanking residue -168 of the mouse rRNA gene are essential to direct efficient transcription in transfected cells and are stimulatory in vitro on closed circular templates. This promoter domain evidently functions by the unprecedented mechanism of terminating polymerase I-directed transcripts. It inhibits transcripts from reading into the initiation region, acting cotranscriptionally to end these RNAs at residue -182 and release them from the template. Most likely, polymerases on tandem ǵenomic rRNA genes are not released upon completing each 40-47S transcript, but traverse the entire spacer to the next promoter-terminator, where they are made available and positioned to favor reinitiation. Through such polymerase recycling, plus the binding of free polymerase, the rDNA promoters could achieve their characteristically high level of transcription.
AB - Sequences flanking residue -168 of the mouse rRNA gene are essential to direct efficient transcription in transfected cells and are stimulatory in vitro on closed circular templates. This promoter domain evidently functions by the unprecedented mechanism of terminating polymerase I-directed transcripts. It inhibits transcripts from reading into the initiation region, acting cotranscriptionally to end these RNAs at residue -182 and release them from the template. Most likely, polymerases on tandem ǵenomic rRNA genes are not released upon completing each 40-47S transcript, but traverse the entire spacer to the next promoter-terminator, where they are made available and positioned to favor reinitiation. Through such polymerase recycling, plus the binding of free polymerase, the rDNA promoters could achieve their characteristically high level of transcription.
UR - http://www.scopus.com/inward/record.url?scp=0022884954&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022884954&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(86)90804-4
DO - 10.1016/0092-8674(86)90804-4
M3 - Article
C2 - 3779844
AN - SCOPUS:0022884954
SN - 0092-8674
VL - 47
SP - 891
EP - 900
JO - Cell
JF - Cell
IS - 6
ER -