A transcriptional reporter of intracellular Ca 2+ in Drosophila

Xiaojing J. Gao, Olena Riabinina, Jiefu Li, Christopher J. Potter, Thomas R. Clandinin, Liqun Luo

Research output: Contribution to journalArticlepeer-review

Abstract

Intracellular Ca 2+ is a widely used neuronal activity indicator. Here we describe a transcriptional reporter of intracellular Ca 2+ (TRIC) in Drosophila that uses a binary expression system to report Ca 2+ -dependent interactions between calmodulin and its target peptide. We found that in vitro assays predicted in vivo properties of TRIC and that TRIC signals in sensory systems depend on neuronal activity. TRIC was able to quantitatively monitor neuronal responses that changed slowly, such as those of neuropeptide F-expressing neurons to sexual deprivation and neuroendocrine pars intercerebralis cells to food and arousal. Furthermore, TRIC-induced expression of a neuronal silencer in nutrient-activated cells enhanced stress resistance, providing a proof of principle that TRIC can be used for circuit manipulation. Thus, TRIC facilitates the monitoring and manipulation of neuronal activity, especially those reflecting slow changes in physiological states that are poorly captured by existing methods. TRIC's modular design should enable optimization and adaptation to other organisms.

Original languageEnglish (US)
Pages (from-to)917-925
Number of pages9
JournalNature neuroscience
Volume18
Issue number6
DOIs
StatePublished - Jun 28 2015

ASJC Scopus subject areas

  • Neuroscience(all)

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