A targeting signal in VAMP regulating transport to synaptic vesicles

Eric Grote, Joe C. Hao, Mark K. Bennett, Regis B. Kelly

Research output: Contribution to journalArticle

Abstract

VAMP is a synaptic vesicle membrane protein required for fusion. Synaptic vesicle targeting was measured for mutants of an epitope-tagged form of VAMP in transfected PC12 cells. A signal within a predicted amphipathic α helix is essential for targeting to synaptic vesicles. Cellubrevin, a nonneural VAMP homolog, contains this signal and is also targeted to synaptic vesicles. Amino acid substitutions within the synaptic vesicle targeting signal either enhance or inhibit sorting of VAMP to synaptic vesicles, but do not affect the ability of VAMP to form complexes with syntaxin and SNAP-25.

Original languageEnglish (US)
Pages (from-to)581-589
Number of pages9
JournalCell
Volume81
Issue number4
DOIs
StatePublished - May 19 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

Grote, E., Hao, J. C., Bennett, M. K., & Kelly, R. B. (1995). A targeting signal in VAMP regulating transport to synaptic vesicles. Cell, 81(4), 581-589. https://doi.org/10.1016/0092-8674(95)90079-9