A systematic bioinformatics approach for selection of epitope-based vaccine targets

Asif M. Khan, Olivo Miotto, A. T. Heiny, Jerome Salmon, K. N. Srinivasan, Eduardo J M Nascimento, Ernesto T A Marques, Vladimir Brusic, Tin Wee Tan, Thomas August

Research output: Contribution to journalArticle

Abstract

Epitope-based vaccines provide a new strategy for prophylactic and therapeutic application of pathogen-specific immunity. A critical requirement of this strategy is the identification and selection of T-cell epitopes that act as vaccine targets. This study describes current methodologies for the selection process, with dengue virus as a model system. A combination of publicly available bioinformatics algorithms and computational tools are used to screen and select antigen sequences as potential T-cell epitopes of supertype human leukocyte antigen (HLA) alleles. The selected sequences are tested for biological function by their activation of T-cells of HLA transgenic mice and of pathogen infected subjects. This approach provides an experimental basis for the design of pathogen specific, T-cell epitope-based vaccines that are targeted to majority of the genetic variants of the pathogen, and are effective for a broad range of differences in human leukocyte antigens among the global human population.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalCellular Immunology
Volume244
Issue number2
DOIs
StatePublished - Dec 2006

Fingerprint

T-Lymphocyte Epitopes
HLA Antigens
Computational Biology
Epitopes
Vaccines
Dengue Virus
Transgenic Mice
Immunity
Research Design
Alleles
T-Lymphocytes
Antigens
Population
Therapeutics

Keywords

  • Altered-ligand effect
  • Bioinformatics
  • Conserved sequences
  • Epitope-based vaccines
  • Immune system
  • Immunological hotspots
  • Information entropy
  • Pathogens
  • Supertypes
  • T-cell epitopes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Khan, A. M., Miotto, O., Heiny, A. T., Salmon, J., Srinivasan, K. N., Nascimento, E. J. M., ... August, T. (2006). A systematic bioinformatics approach for selection of epitope-based vaccine targets. Cellular Immunology, 244(2), 141-147. https://doi.org/10.1016/j.cellimm.2007.02.005

A systematic bioinformatics approach for selection of epitope-based vaccine targets. / Khan, Asif M.; Miotto, Olivo; Heiny, A. T.; Salmon, Jerome; Srinivasan, K. N.; Nascimento, Eduardo J M; Marques, Ernesto T A; Brusic, Vladimir; Tan, Tin Wee; August, Thomas.

In: Cellular Immunology, Vol. 244, No. 2, 12.2006, p. 141-147.

Research output: Contribution to journalArticle

Khan, AM, Miotto, O, Heiny, AT, Salmon, J, Srinivasan, KN, Nascimento, EJM, Marques, ETA, Brusic, V, Tan, TW & August, T 2006, 'A systematic bioinformatics approach for selection of epitope-based vaccine targets', Cellular Immunology, vol. 244, no. 2, pp. 141-147. https://doi.org/10.1016/j.cellimm.2007.02.005
Khan AM, Miotto O, Heiny AT, Salmon J, Srinivasan KN, Nascimento EJM et al. A systematic bioinformatics approach for selection of epitope-based vaccine targets. Cellular Immunology. 2006 Dec;244(2):141-147. https://doi.org/10.1016/j.cellimm.2007.02.005
Khan, Asif M. ; Miotto, Olivo ; Heiny, A. T. ; Salmon, Jerome ; Srinivasan, K. N. ; Nascimento, Eduardo J M ; Marques, Ernesto T A ; Brusic, Vladimir ; Tan, Tin Wee ; August, Thomas. / A systematic bioinformatics approach for selection of epitope-based vaccine targets. In: Cellular Immunology. 2006 ; Vol. 244, No. 2. pp. 141-147.
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