A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function

Jason Constantineau, Erin Greason, Michael West, Megan Filbin, Jeffrey S. Kieft, Martha Z. Carletti, Lane K. Christenson, Annabelle Rodriguez

Research output: Contribution to journalArticle

Abstract

Objective: A synonymous variant within scavenger receptor class B type I gene (SCARB1), exon 8 rs5888, has been associated with altered lipid levels and cardiovascular risk in humans. The objective was to determine if rs5888 decreased SR-BI protein expression and function in vitro. Methods: SR-BI RNA secondary structure, turnover, polysomal distribution and protein expression were examined in COS cells transfected with wild-type or rs5888-SR-BI plasmids by selective 2′-hydroxyl acylation and primer extension assays, actinomycin D inhibition, polysomal profiling, and western blotting. SR-BI function in murine macrophages stably expressing wild-type or rs5888-SR-BI was assessed by measuring the specific cell association of 125I,3H-cholesteryl ester (CE) radiolabeled HDL. Results: Rs5888 changed RNA secondary structure and led to marked differences in the polysomal profiles compared with wild-type transcript (p125I (p3H-CE (p

LanguageEnglish (US)
Pages177-182
Number of pages6
JournalAtherosclerosis
Volume210
Issue number1
DOIs
StatePublished - May 2010

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CD36 Antigens
MHC Class I Genes
RNA
Acylation
Cholesterol Esters
COS Cells
Dactinomycin
Hydroxyl Radical
Exons
Plasmids
Western Blotting
Macrophages
Lipids
Proteins
HDL cholesteryl ester
In Vitro Techniques

Keywords

  • Atherosclerosis
  • HDL
  • SCARB1
  • SNPs
  • SR-BI
  • Synonymous

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function. / Constantineau, Jason; Greason, Erin; West, Michael; Filbin, Megan; Kieft, Jeffrey S.; Carletti, Martha Z.; Christenson, Lane K.; Rodriguez, Annabelle.

In: Atherosclerosis, Vol. 210, No. 1, 05.2010, p. 177-182.

Research output: Contribution to journalArticle

Constantineau, J, Greason, E, West, M, Filbin, M, Kieft, JS, Carletti, MZ, Christenson, LK & Rodriguez, A 2010, 'A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function' Atherosclerosis, vol. 210, no. 1, pp. 177-182. DOI: 10.1016/j.atherosclerosis.2009.11.029
Constantineau J, Greason E, West M, Filbin M, Kieft JS, Carletti MZ et al. A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function. Atherosclerosis. 2010 May;210(1):177-182. Available from, DOI: 10.1016/j.atherosclerosis.2009.11.029
Constantineau, Jason ; Greason, Erin ; West, Michael ; Filbin, Megan ; Kieft, Jeffrey S. ; Carletti, Martha Z. ; Christenson, Lane K. ; Rodriguez, Annabelle. / A synonymous variant in scavenger receptor, class B, type I gene is associated with lower SR-BI protein expression and function. In: Atherosclerosis. 2010 ; Vol. 210, No. 1. pp. 177-182
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