A survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries

Stefan Riedel, Nicholas Boire, Kathryn Anne Carson, Aravinda Vadlamudi, Joshua Khuvis, Vivek Vadlamudi, Vajini Atukorale, Victoria A.A. Riedel, Nicole M Parrish

Research output: Contribution to journalArticle

Abstract

In recent years, the rise in antimicrobial resistance (AR) in the healthcare setting as well as the environment has been recognized as a growing public health problem. The Chesapeake Bay (CB) and its upper tributaries (UT) is a large and biologically diverse estuary. This pilot study evaluated the presence of AR of gram-negative bacteria isolated from water samples collected at various sites of the Chesapeake Bay. Bacterial organisms were identified and antimicrobial susceptibility testing was performed by phenotypic and genotypic methods. Ninety-two distinctly different gram-negative bacteria were identified; Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Escherichia coli were most often isolated. Serratia marcescens was more frequently isolated in samples from the UT compared to the CB. Antimicrobial resistance was more frequently detected in organisms from the CB by phenotypic and genotypic methods. Antimicrobial resistance to ampicillin, imipenem, tetracycline, and chloramphenicol were the most frequently observed resistance patterns. ACT-1, CMY, and SHV genes were the most frequently detected resistance genes, with predominance in organism isolated from the CB. The results from this study emphasize the importance for further developing comprehensive surveillance programs of AR in bacterial isolates in the various environments, such as recreational and other water systems.

Original languageEnglish (US)
Article numbere839
JournalMicrobiologyOpen
DOIs
StatePublished - Jan 1 2019

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Enterobacteriaceae
Serratia marcescens
Gram-Negative Bacteria
Ampicillin Resistance
Enterobacter aerogenes
Enterobacter cloacae
Estuaries
Water
Imipenem
Klebsiella pneumoniae
Chloramphenicol
Tetracycline
Genes
Public Health
Surveys and Questionnaires
Escherichia coli
Delivery of Health Care

Keywords

  • antibiotic pollution
  • antimicrobial resistance
  • Chesapeake Bay
  • Enterobacteriaceae
  • One Health

ASJC Scopus subject areas

  • Microbiology

Cite this

A survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries. / Riedel, Stefan; Boire, Nicholas; Carson, Kathryn Anne; Vadlamudi, Aravinda; Khuvis, Joshua; Vadlamudi, Vivek; Atukorale, Vajini; Riedel, Victoria A.A.; Parrish, Nicole M.

In: MicrobiologyOpen, 01.01.2019.

Research output: Contribution to journalArticle

Riedel, Stefan ; Boire, Nicholas ; Carson, Kathryn Anne ; Vadlamudi, Aravinda ; Khuvis, Joshua ; Vadlamudi, Vivek ; Atukorale, Vajini ; Riedel, Victoria A.A. ; Parrish, Nicole M. / A survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries. In: MicrobiologyOpen. 2019.
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