A surface tension magnetophoretic device for rare cell isolation and characterization

Emma E. van der Toom, James E. Verdone, Changhan Jun, Doru Petrisor, Sunghwan Lim, Jean J M C H de la Rosette, Theo M. de Reijke, Michael Gorin, Kenneth Pienta, Dan Stoianovici

Research output: Contribution to journalArticle

Abstract

The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

Original languageEnglish (US)
Article number22
JournalMedical Oncology
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2017

Keywords

  • Circulating tumor cells
  • Magnetophoresis
  • Micro-fluidic device
  • Prostate cancer
  • Rare cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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