A surface tension magnetophoretic device for rare cell isolation and characterization

Emma E. van der Toom, James E. Verdone, Changhan Jun, Doru Petrisor, Sunghwan Lim, Jean J M C H de la Rosette, Theo M. de Reijke, Michael Gorin, Kenneth Pienta, Dan Stoianovici

Research output: Contribution to journalArticle

Abstract

The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

LanguageEnglish (US)
Article number22
JournalMedical Oncology
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Surface Tension
Cell Separation
Equipment and Supplies
Staining and Labeling
Fluorescent Antibody Technique
Neoplasms
Biopsy
Costs and Cost Analysis
Antibodies
Population

Keywords

  • Circulating tumor cells
  • Magnetophoresis
  • Micro-fluidic device
  • Prostate cancer
  • Rare cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

A surface tension magnetophoretic device for rare cell isolation and characterization. / van der Toom, Emma E.; Verdone, James E.; Jun, Changhan; Petrisor, Doru; Lim, Sunghwan; de la Rosette, Jean J M C H; de Reijke, Theo M.; Gorin, Michael; Pienta, Kenneth; Stoianovici, Dan.

In: Medical Oncology, Vol. 34, No. 2, 22, 01.02.2017.

Research output: Contribution to journalArticle

van der Toom, Emma E. ; Verdone, James E. ; Jun, Changhan ; Petrisor, Doru ; Lim, Sunghwan ; de la Rosette, Jean J M C H ; de Reijke, Theo M. ; Gorin, Michael ; Pienta, Kenneth ; Stoianovici, Dan. / A surface tension magnetophoretic device for rare cell isolation and characterization. In: Medical Oncology. 2017 ; Vol. 34, No. 2.
@article{4d39ed105f904291bb918b0cb5326355,
title = "A surface tension magnetophoretic device for rare cell isolation and characterization",
abstract = "The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.",
keywords = "Circulating tumor cells, Magnetophoresis, Micro-fluidic device, Prostate cancer, Rare cells",
author = "{van der Toom}, {Emma E.} and Verdone, {James E.} and Changhan Jun and Doru Petrisor and Sunghwan Lim and {de la Rosette}, {Jean J M C H} and {de Reijke}, {Theo M.} and Michael Gorin and Kenneth Pienta and Dan Stoianovici",
year = "2017",
month = "2",
day = "1",
doi = "10.1007/s12032-016-0877-y",
language = "English (US)",
volume = "34",
journal = "Medical Oncology",
issn = "1357-0560",
publisher = "Humana Press",
number = "2",

}

TY - JOUR

T1 - A surface tension magnetophoretic device for rare cell isolation and characterization

AU - van der Toom, Emma E.

AU - Verdone, James E.

AU - Jun, Changhan

AU - Petrisor, Doru

AU - Lim, Sunghwan

AU - de la Rosette, Jean J M C H

AU - de Reijke, Theo M.

AU - Gorin, Michael

AU - Pienta, Kenneth

AU - Stoianovici, Dan

PY - 2017/2/1

Y1 - 2017/2/1

N2 - The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

AB - The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

KW - Circulating tumor cells

KW - Magnetophoresis

KW - Micro-fluidic device

KW - Prostate cancer

KW - Rare cells

UR - http://www.scopus.com/inward/record.url?scp=85008391980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008391980&partnerID=8YFLogxK

U2 - 10.1007/s12032-016-0877-y

DO - 10.1007/s12032-016-0877-y

M3 - Article

VL - 34

JO - Medical Oncology

T2 - Medical Oncology

JF - Medical Oncology

SN - 1357-0560

IS - 2

M1 - 22

ER -