A surface tension magnetophoretic device for rare cell isolation and characterization

Emma E. van der Toom, James E. Verdone, Changhan Jun, Doru Petrisor, Sunghwan Lim, Jean J M C H de la Rosette, Theo M. de Reijke, Michael A. Gorin, Kenneth J. Pienta, Dan Stoianovici

Research output: Research - peer-reviewArticle

Abstract

The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a ‘real-time liquid biopsy’. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive ‘lab on a chip’ device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.

LanguageEnglish (US)
Article number22
JournalMedical Oncology
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Surface Tension
Cell Separation
Equipment and Supplies
Staining and Labeling
Neoplasms
Fluorescent Antibody Technique
Biopsy
Costs and Cost Analysis
Antibodies
Population
Therapeutics

Keywords

  • Circulating tumor cells
  • Magnetophoresis
  • Micro-fluidic device
  • Prostate cancer
  • Rare cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

van der Toom, E. E., Verdone, J. E., Jun, C., Petrisor, D., Lim, S., de la Rosette, J. J. M. C. H., ... Stoianovici, D. (2017). A surface tension magnetophoretic device for rare cell isolation and characterization. Medical Oncology, 34(2), [22]. DOI: 10.1007/s12032-016-0877-y

A surface tension magnetophoretic device for rare cell isolation and characterization. / van der Toom, Emma E.; Verdone, James E.; Jun, Changhan; Petrisor, Doru; Lim, Sunghwan; de la Rosette, Jean J M C H; de Reijke, Theo M.; Gorin, Michael A.; Pienta, Kenneth J.; Stoianovici, Dan.

In: Medical Oncology, Vol. 34, No. 2, 22, 01.02.2017.

Research output: Research - peer-reviewArticle

van der Toom, EE, Verdone, JE, Jun, C, Petrisor, D, Lim, S, de la Rosette, JJMCH, de Reijke, TM, Gorin, MA, Pienta, KJ & Stoianovici, D 2017, 'A surface tension magnetophoretic device for rare cell isolation and characterization' Medical Oncology, vol 34, no. 2, 22. DOI: 10.1007/s12032-016-0877-y
van der Toom EE, Verdone JE, Jun C, Petrisor D, Lim S, de la Rosette JJMCH et al. A surface tension magnetophoretic device for rare cell isolation and characterization. Medical Oncology. 2017 Feb 1;34(2). 22. Available from, DOI: 10.1007/s12032-016-0877-y
van der Toom, Emma E. ; Verdone, James E. ; Jun, Changhan ; Petrisor, Doru ; Lim, Sunghwan ; de la Rosette, Jean J M C H ; de Reijke, Theo M. ; Gorin, Michael A. ; Pienta, Kenneth J. ; Stoianovici, Dan. / A surface tension magnetophoretic device for rare cell isolation and characterization. In: Medical Oncology. 2017 ; Vol. 34, No. 2.
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