A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix

Bert Vogelstein; Brett F. Hunt

doi:10.1016/0006-291X(82)91099-3

A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix

Bert Vogelstein, Brett F. Hunt

School of Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We have assessed whether antigenic proteins associated with small nuclear ribonucleoprotein particles (snRNP) are associated with the nuclear matrix. Immunofluorescence studies showed that a subset of these particles (those reactive with anti-Sm antisera) were associated with the nuclear matrix, while a different set of particles (those reactive with anti-La antisera) were not associated with the nuclear matrix. Immunoprecipitation experiments showed that three specific polypeptide components of the snRNP reactive with the anti-Sm antisera were significantly enriched in nuclear matrix proteins.

Original language	English (US)
Pages (from-to)	1224-1232
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	105
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(82)91099-3
State	Published - Apr 14 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(82)91099-3

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title = "A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix",

abstract = "We have assessed whether antigenic proteins associated with small nuclear ribonucleoprotein particles (snRNP) are associated with the nuclear matrix. Immunofluorescence studies showed that a subset of these particles (those reactive with anti-Sm antisera) were associated with the nuclear matrix, while a different set of particles (those reactive with anti-La antisera) were not associated with the nuclear matrix. Immunoprecipitation experiments showed that three specific polypeptide components of the snRNP reactive with the anti-Sm antisera were significantly enriched in nuclear matrix proteins.",

author = "Bert Vogelstein and Hunt, {Brett F.}",

note = "Funding Information: ACKNOWLEDGEMENTS: The authors are grateful to Dr. Morris Reichlin (Oklahoma Medical Research Foundation) for generously supplying us with the antisera in this study. We also wish to thank Ms. M.A. Egan for editorial assistance. This work was supported by NIH grant #CA06973 and a gift from Bristol-Myers co.",

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T1 - A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix

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AU - Hunt, Brett F.

N1 - Funding Information: ACKNOWLEDGEMENTS: The authors are grateful to Dr. Morris Reichlin (Oklahoma Medical Research Foundation) for generously supplying us with the antisera in this study. We also wish to thank Ms. M.A. Egan for editorial assistance. This work was supported by NIH grant #CA06973 and a gift from Bristol-Myers co.

PY - 1982/4/14

Y1 - 1982/4/14

N2 - We have assessed whether antigenic proteins associated with small nuclear ribonucleoprotein particles (snRNP) are associated with the nuclear matrix. Immunofluorescence studies showed that a subset of these particles (those reactive with anti-Sm antisera) were associated with the nuclear matrix, while a different set of particles (those reactive with anti-La antisera) were not associated with the nuclear matrix. Immunoprecipitation experiments showed that three specific polypeptide components of the snRNP reactive with the anti-Sm antisera were significantly enriched in nuclear matrix proteins.

AB - We have assessed whether antigenic proteins associated with small nuclear ribonucleoprotein particles (snRNP) are associated with the nuclear matrix. Immunofluorescence studies showed that a subset of these particles (those reactive with anti-Sm antisera) were associated with the nuclear matrix, while a different set of particles (those reactive with anti-La antisera) were not associated with the nuclear matrix. Immunoprecipitation experiments showed that three specific polypeptide components of the snRNP reactive with the anti-Sm antisera were significantly enriched in nuclear matrix proteins.

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A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix

Abstract

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