A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation

Tatiana Gerasimova, Changying Guo, Amalendu Ghosh, Xiang Qiu, Lindsey Montefiori, Jiyoti Verma-Gaur, Nancy M. Choi, Ann J. Feeney, Ranjan Sen

Research output: Contribution to journalArticle

Abstract

Conformation of antigen receptor gene loci spatially juxtaposes rearranging gene segments in the appropriate cell lineage and developmental stage. We describe a three-step pathway that establishes the structure of the 2.8-Mb immunoglobulin heavy chain gene (IgH) locus in pro-B cells. Each step uses a different transcription factor and leads to increasing levels of structural organization. CTCF mediates one level of compaction that folds the locus into several 250- to 400-kb subdomains, and Pax5 further compacts the 2-Mb region that encodes variable (VH) gene segments. The 5′ and 3′ domains are brought together by the transcription factor YY1 to establish the configuration within which gene recombination initiates. Such stepwise mechanisms may apply more generally to establish regulatory fine structure within megabase-sized topologically associated domains.

Original languageEnglish (US)
Pages (from-to)1683-1695
Number of pages13
JournalGenes & development
Volume29
Issue number16
DOIs
StatePublished - Aug 15 2015
Externally publishedYes

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Keywords

  • Architectural proteins
  • Chromosome conformation
  • Looping

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Gerasimova, T., Guo, C., Ghosh, A., Qiu, X., Montefiori, L., Verma-Gaur, J., Choi, N. M., Feeney, A. J., & Sen, R. (2015). A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation. Genes & development, 29(16), 1683-1695. https://doi.org/10.1101/gad.263871.115.