TY - GEN
T1 - A statistical model of proteolytic digestion
AU - Wang, I. J.
AU - Diehl, C. P.
AU - Pineda, F. J.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We present a stochastic model of proteolytic digestion of a proteome, assuming the distribution of parent protein lengths in the proteome, the relative abundances of the 20 amino acids in the proteome, and the digestion "rules" of the enzyme used in the digestion. We derived a closed form expression for the fragment mass distribution for a large class of enzymes including the widely used trypsin. The expression uses the distribution of lengths in a mixture of proteins taken from a proteome, as well as the relative abundances of the 20 amino acids in the proteome. The agreement between theory and the in silica digest is excellent.
AB - We present a stochastic model of proteolytic digestion of a proteome, assuming the distribution of parent protein lengths in the proteome, the relative abundances of the 20 amino acids in the proteome, and the digestion "rules" of the enzyme used in the digestion. We derived a closed form expression for the fragment mass distribution for a large class of enzymes including the widely used trypsin. The expression uses the distribution of lengths in a mixture of proteins taken from a proteome, as well as the relative abundances of the 20 amino acids in the proteome. The agreement between theory and the in silica digest is excellent.
UR - http://www.scopus.com/inward/record.url?scp=26444548436&partnerID=8YFLogxK
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U2 - 10.1109/CSB.2003.1227390
DO - 10.1109/CSB.2003.1227390
M3 - Conference contribution
AN - SCOPUS:26444548436
T3 - Proceedings of the 2003 IEEE Bioinformatics Conference, CSB 2003
SP - 506
EP - 508
BT - Proceedings of the 2003 IEEE Bioinformatics Conference, CSB 2003
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2nd International IEEE Computer Society Computational Systems Bioinformatics Conference, CSB 2003
Y2 - 11 August 2003 through 14 August 2003
ER -