TY - JOUR
T1 - A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I
AU - Cizewski, Valeria
AU - Sollner-webb, Barbara
N1 - Funding Information:
ACgWWLEBgBBWTS We thank the American Cancer Society for snpport of this cork. This work was also aided by a Basil O'Connor Starter Research Grant from the March of Dimes Birth Defects Foundation.
PY - 1983/10/25
Y1 - 1983/10/25
N2 - Ribosomal RNA is synthesized from template molecules that are activated by a stable association with essential transcription factors. This activated template assembles prior to the onset of transcription as a preinitiation complex and factors remain firmly attached during active elongation as well. Sequential addition of differently marked rRNA genes to an S-100 mouse cell extract shows that the DNA binding factors of the stable complex are present in limiting quantities. They associate rapidly with template molecules and the resultant transcription complex remains intact over prolonged periods of incubation in the presence of competitor DNA. The resistance of the stable complex to the usual inhibitory effect of high DNA concentration suggests that more than one DNA binding factor recognizes the rDNA promoter region and is needed to direct faithful transcription. Finally, although the stable complex is specific for the rRNA initiation region, added vector sequences can neutralize nonspecific DNA binding components that are also present in the cell extract. This lowers the requirement for rDNA template and demonstrates that each activated rRNA gene can direct at least 10 rounds of elongation and reinitiation.
AB - Ribosomal RNA is synthesized from template molecules that are activated by a stable association with essential transcription factors. This activated template assembles prior to the onset of transcription as a preinitiation complex and factors remain firmly attached during active elongation as well. Sequential addition of differently marked rRNA genes to an S-100 mouse cell extract shows that the DNA binding factors of the stable complex are present in limiting quantities. They associate rapidly with template molecules and the resultant transcription complex remains intact over prolonged periods of incubation in the presence of competitor DNA. The resistance of the stable complex to the usual inhibitory effect of high DNA concentration suggests that more than one DNA binding factor recognizes the rDNA promoter region and is needed to direct faithful transcription. Finally, although the stable complex is specific for the rRNA initiation region, added vector sequences can neutralize nonspecific DNA binding components that are also present in the cell extract. This lowers the requirement for rDNA template and demonstrates that each activated rRNA gene can direct at least 10 rounds of elongation and reinitiation.
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U2 - 10.1093/nar/11.20.7043
DO - 10.1093/nar/11.20.7043
M3 - Article
C2 - 6314273
AN - SCOPUS:0021112676
SN - 0305-1048
VL - 11
SP - 7043
EP - 7056
JO - Nucleic acids research
JF - Nucleic acids research
IS - 20
ER -