A specific antigen-detection ELISA for the diagnosis of human neurocysticercosis

H. H. Garcia, L. J S Harrison, R. M E Parkhouse, T. Montenegro, S. M. Martinez, V. C W Tsang, Robert H Gilman, M. Tovar, S. Rodriguez, M. Alvarado, J. Nunez, L. Portilla, A. Valencia, L. Torres, M. Verastegui, G. Herrera, Armandoe Gonzalez, C. Gavidia, N. Falcon, T. BernalC. A W Evans

Research output: Contribution to journalArticle

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detection of antigen secreted by viable Taenia solium metacestodes (Ag-ELISA) was applied to 43 pre-treatment and 47 follow-up cerebrospinal fluid (CSF) samples from Peruvian patients with neurocysticercosis demonstrated by computed tomography and enzyme-linked immunoelectrotransfer blot assay. The sensitivity of the assay was 86%. Negative pretreatment results in the Ag-ELISA test were restricted to patients with only a single live cyst or only enhancing lesions. Patients with hydrocephalus had higher levels of circulating antigen. There was no difference between antigen levels in CSF taken before and immediately after treatment (day 14). Levels of parasite antigen were significantly positively correlated with the number of live cysts detected by tomography and were also proportional to the number and intensity of antibody reactions recognized by the immunoblot diagnostic test. In contrast, there was a negative correlation with the number of enhancing lesions revealed by tomography, supporting the hypothesis that enhancing lesions correspond to a terminal, moribund stage of the parasite. The use of antigen-detection tests specific for viable metacestodes has immediate utility in the clinical context, not only providing important information on the viability of the parasites but also leading to an improved understanding of the pathogenesis of neurocysticercosis before and after drug treatment.

Original languageEnglish (US)
Pages (from-to)411-414
Number of pages4
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume92
Issue number4
DOIs
StatePublished - 1998

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Neurocysticercosis
Enzyme-Linked Immunosorbent Assay
Antigens
Parasites
Tomography
Cerebrospinal Fluid
Cysts
Taenia solium
Hydrocephalus
Routine Diagnostic Tests
Therapeutics
Antibodies
Enzymes
Pharmaceutical Preparations

Keywords

  • Antigen detection
  • Diagnosis
  • Enzyme-linked immunosorbent assay
  • Neurocysticercosis
  • Taenia solium

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

Cite this

Garcia, H. H., Harrison, L. J. S., Parkhouse, R. M. E., Montenegro, T., Martinez, S. M., Tsang, V. C. W., ... Evans, C. A. W. (1998). A specific antigen-detection ELISA for the diagnosis of human neurocysticercosis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 92(4), 411-414. https://doi.org/10.1016/S0035-9203(98)91069-0

A specific antigen-detection ELISA for the diagnosis of human neurocysticercosis. / Garcia, H. H.; Harrison, L. J S; Parkhouse, R. M E; Montenegro, T.; Martinez, S. M.; Tsang, V. C W; Gilman, Robert H; Tovar, M.; Rodriguez, S.; Alvarado, M.; Nunez, J.; Portilla, L.; Valencia, A.; Torres, L.; Verastegui, M.; Herrera, G.; Gonzalez, Armandoe; Gavidia, C.; Falcon, N.; Bernal, T.; Evans, C. A W.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 92, No. 4, 1998, p. 411-414.

Research output: Contribution to journalArticle

Garcia, HH, Harrison, LJS, Parkhouse, RME, Montenegro, T, Martinez, SM, Tsang, VCW, Gilman, RH, Tovar, M, Rodriguez, S, Alvarado, M, Nunez, J, Portilla, L, Valencia, A, Torres, L, Verastegui, M, Herrera, G, Gonzalez, A, Gavidia, C, Falcon, N, Bernal, T & Evans, CAW 1998, 'A specific antigen-detection ELISA for the diagnosis of human neurocysticercosis', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 92, no. 4, pp. 411-414. https://doi.org/10.1016/S0035-9203(98)91069-0
Garcia, H. H. ; Harrison, L. J S ; Parkhouse, R. M E ; Montenegro, T. ; Martinez, S. M. ; Tsang, V. C W ; Gilman, Robert H ; Tovar, M. ; Rodriguez, S. ; Alvarado, M. ; Nunez, J. ; Portilla, L. ; Valencia, A. ; Torres, L. ; Verastegui, M. ; Herrera, G. ; Gonzalez, Armandoe ; Gavidia, C. ; Falcon, N. ; Bernal, T. ; Evans, C. A W. / A specific antigen-detection ELISA for the diagnosis of human neurocysticercosis. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 1998 ; Vol. 92, No. 4. pp. 411-414.
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AU - Montenegro, T.

AU - Martinez, S. M.

AU - Tsang, V. C W

AU - Gilman, Robert H

AU - Tovar, M.

AU - Rodriguez, S.

AU - Alvarado, M.

AU - Nunez, J.

AU - Portilla, L.

AU - Valencia, A.

AU - Torres, L.

AU - Verastegui, M.

AU - Herrera, G.

AU - Gonzalez, Armandoe

AU - Gavidia, C.

AU - Falcon, N.

AU - Bernal, T.

AU - Evans, C. A W

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