A soluble 17β-hydroxysteroid dehydrogenase from human placenta. The binding of pyridine nucleotides and steroids

Joseph Jarabak, George Henry Sack

Research output: Contribution to journalArticle

Abstract

The molecular weight of the 17β-hydroxysteroid dehydrogenase of human placenta, estimated by gel filtration and reduced nicotinamide-adenine dinucleotide phosphate titration, is 62,000-65,000. Fluorescence measurements indicate that the Michaelis constants of nicotinamide-adenine dinucleotide phosphate and reduced nicotinamide-adenine dinucleotide phosphate are 0.89 and 0.83 μM, respectively, and that the dissociation constants of these pyridine nucleotides are 51 and 46 mμM, respectively. When the enzyme is cooled in the presence of 1% glycerol, it loses its activity rapidly and this loss is paralleled by a decrease in the ability of the enzyme to bind reduced nicotinamide-adenine dinucleotide phosphate. Because of the rather broad substrate specificity of this enzyme, various compounds were examined for their effect on 17β-estradiol oxidation. Structural analogs of 17β-estradiol (17-desoxyestradiol and 17α-estradiol), as well as certain other steroid hormones (androst-4-en-3,17-dione and progesterone) and nonsteroidal compounds (2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethane and diethylstilbestrol), were found to be competitive inhibitors of 17β-estradiol oxidation, while corticosteroids did not inhibit it. Binding studies by ultracentrifugation indicate a single binding site on the enzyme for 17β-estradiol and estrone. When examined by gel filtration the binding of 17β-estradiol to the enzyme was found to be reduced by diethylstilbestrol as well as by a synthetic estrogen antagonist, 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphthyl)phenoxy]ethyl)pyrrolidine. Conditions which inactivate the enzyme (cold sodium dodecyl sulfate, p-hydroxymercuribenzoate, and guanidine hydrochloride) also reduce its estrogen binding capacity.

Original languageEnglish (US)
Pages (from-to)2203-2212
Number of pages10
JournalBiochemistry®
Volume8
Issue number5
StatePublished - 1969

Fingerprint

Placenta
Estradiol
Nucleotides
Steroids
NADP
Enzymes
Diethylstilbestrol
Gel Chromatography
Gels
Steroid hormones
Estradiol Congeners
Estrogen Antagonists
Oxidation
Estrone
Ultracentrifugation
Guanidine
Corrosion inhibitors
Substrate Specificity
Titration
Sodium Dodecyl Sulfate

ASJC Scopus subject areas

  • Biochemistry

Cite this

A soluble 17β-hydroxysteroid dehydrogenase from human placenta. The binding of pyridine nucleotides and steroids. / Jarabak, Joseph; Sack, George Henry.

In: Biochemistry®, Vol. 8, No. 5, 1969, p. 2203-2212.

Research output: Contribution to journalArticle

@article{09a5acb4cc78450086a9956a5cf852a0,
title = "A soluble 17β-hydroxysteroid dehydrogenase from human placenta. The binding of pyridine nucleotides and steroids",
abstract = "The molecular weight of the 17β-hydroxysteroid dehydrogenase of human placenta, estimated by gel filtration and reduced nicotinamide-adenine dinucleotide phosphate titration, is 62,000-65,000. Fluorescence measurements indicate that the Michaelis constants of nicotinamide-adenine dinucleotide phosphate and reduced nicotinamide-adenine dinucleotide phosphate are 0.89 and 0.83 μM, respectively, and that the dissociation constants of these pyridine nucleotides are 51 and 46 mμM, respectively. When the enzyme is cooled in the presence of 1{\%} glycerol, it loses its activity rapidly and this loss is paralleled by a decrease in the ability of the enzyme to bind reduced nicotinamide-adenine dinucleotide phosphate. Because of the rather broad substrate specificity of this enzyme, various compounds were examined for their effect on 17β-estradiol oxidation. Structural analogs of 17β-estradiol (17-desoxyestradiol and 17α-estradiol), as well as certain other steroid hormones (androst-4-en-3,17-dione and progesterone) and nonsteroidal compounds (2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethane and diethylstilbestrol), were found to be competitive inhibitors of 17β-estradiol oxidation, while corticosteroids did not inhibit it. Binding studies by ultracentrifugation indicate a single binding site on the enzyme for 17β-estradiol and estrone. When examined by gel filtration the binding of 17β-estradiol to the enzyme was found to be reduced by diethylstilbestrol as well as by a synthetic estrogen antagonist, 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphthyl)phenoxy]ethyl)pyrrolidine. Conditions which inactivate the enzyme (cold sodium dodecyl sulfate, p-hydroxymercuribenzoate, and guanidine hydrochloride) also reduce its estrogen binding capacity.",
author = "Joseph Jarabak and Sack, {George Henry}",
year = "1969",
language = "English (US)",
volume = "8",
pages = "2203--2212",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - A soluble 17β-hydroxysteroid dehydrogenase from human placenta. The binding of pyridine nucleotides and steroids

AU - Jarabak, Joseph

AU - Sack, George Henry

PY - 1969

Y1 - 1969

N2 - The molecular weight of the 17β-hydroxysteroid dehydrogenase of human placenta, estimated by gel filtration and reduced nicotinamide-adenine dinucleotide phosphate titration, is 62,000-65,000. Fluorescence measurements indicate that the Michaelis constants of nicotinamide-adenine dinucleotide phosphate and reduced nicotinamide-adenine dinucleotide phosphate are 0.89 and 0.83 μM, respectively, and that the dissociation constants of these pyridine nucleotides are 51 and 46 mμM, respectively. When the enzyme is cooled in the presence of 1% glycerol, it loses its activity rapidly and this loss is paralleled by a decrease in the ability of the enzyme to bind reduced nicotinamide-adenine dinucleotide phosphate. Because of the rather broad substrate specificity of this enzyme, various compounds were examined for their effect on 17β-estradiol oxidation. Structural analogs of 17β-estradiol (17-desoxyestradiol and 17α-estradiol), as well as certain other steroid hormones (androst-4-en-3,17-dione and progesterone) and nonsteroidal compounds (2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethane and diethylstilbestrol), were found to be competitive inhibitors of 17β-estradiol oxidation, while corticosteroids did not inhibit it. Binding studies by ultracentrifugation indicate a single binding site on the enzyme for 17β-estradiol and estrone. When examined by gel filtration the binding of 17β-estradiol to the enzyme was found to be reduced by diethylstilbestrol as well as by a synthetic estrogen antagonist, 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphthyl)phenoxy]ethyl)pyrrolidine. Conditions which inactivate the enzyme (cold sodium dodecyl sulfate, p-hydroxymercuribenzoate, and guanidine hydrochloride) also reduce its estrogen binding capacity.

AB - The molecular weight of the 17β-hydroxysteroid dehydrogenase of human placenta, estimated by gel filtration and reduced nicotinamide-adenine dinucleotide phosphate titration, is 62,000-65,000. Fluorescence measurements indicate that the Michaelis constants of nicotinamide-adenine dinucleotide phosphate and reduced nicotinamide-adenine dinucleotide phosphate are 0.89 and 0.83 μM, respectively, and that the dissociation constants of these pyridine nucleotides are 51 and 46 mμM, respectively. When the enzyme is cooled in the presence of 1% glycerol, it loses its activity rapidly and this loss is paralleled by a decrease in the ability of the enzyme to bind reduced nicotinamide-adenine dinucleotide phosphate. Because of the rather broad substrate specificity of this enzyme, various compounds were examined for their effect on 17β-estradiol oxidation. Structural analogs of 17β-estradiol (17-desoxyestradiol and 17α-estradiol), as well as certain other steroid hormones (androst-4-en-3,17-dione and progesterone) and nonsteroidal compounds (2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1-dichloroethane and diethylstilbestrol), were found to be competitive inhibitors of 17β-estradiol oxidation, while corticosteroids did not inhibit it. Binding studies by ultracentrifugation indicate a single binding site on the enzyme for 17β-estradiol and estrone. When examined by gel filtration the binding of 17β-estradiol to the enzyme was found to be reduced by diethylstilbestrol as well as by a synthetic estrogen antagonist, 1-(2-[p-(3,4-dihydro-6-methoxy-2-phenyl-1-naphthyl)phenoxy]ethyl)pyrrolidine. Conditions which inactivate the enzyme (cold sodium dodecyl sulfate, p-hydroxymercuribenzoate, and guanidine hydrochloride) also reduce its estrogen binding capacity.

UR - http://www.scopus.com/inward/record.url?scp=0014512087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014512087&partnerID=8YFLogxK

M3 - Article

C2 - 4389089

AN - SCOPUS:0014512087

VL - 8

SP - 2203

EP - 2212

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 5

ER -